Generation of free radicals is thought to mediate the cytotoxic action of alloxan on the pancreatic beta-cell. In this investigation, the early effects of alloxan on cell function were studied. When INS-1D insulinoma cells were exposed to alloxan (1 mM) for 45 min followed by a 3-hr recovery period, the drug increased basal insulin release while abolishing the effect of glucose in static incuba...

Dehydroascorbic acid has been shown to produce diabetes (1, 2). In this respect it is similar to alloxan (3). Whereas alloxan has not yet been shown to occur physiologically, dehydroascorbic acid is accepted as occurring under physiological conditions in the cells of animals (4). Therefore, it is important to study the factors that are known to affect alloxan diabetes in order to determine thei...

Wistar rats were given 20 ppm fluoride in drinking water, or single administration of 115 mg/kg alloxan i.m. to induce diabetes, or single administration of 115 mg/kg alloxan i.m. followed by 20 ppm fluoride for 31 days. Blood sugar level increased in rats given alloxan and alloxan + fluoride. Body weight gain in rats given alloxan + fluoride decreased significantly compared to other groups. De...

The effect of various anti-microtubular agents on alloxan stimulation and inhibition of a subsequent glucose-induced insulin release was investigated using in vitro incubation and perifusion of rat isolated islets of Langerhans. Five minute exposure of islets to alloxan (20 mg/dl) induced a transient burst of insulin release which was eliminated by the pretreatment of islets by heavy water (D20...

Clinical and experimental evidence suggests that hyperglycaemia is responsible for the oxidative stress in diabetes mellitus. The study was designed to investigate the comparative effects of probiotic and vitamin C (Vit-C) treatments on hyperglycaemia, oxidative stress and dyslipidaemia in alloxan-induced diabetic rats. Type 1 diabetes (T1DM) was induced in male Wistar rats by a single intraper...

To evaluate the participation of mitochondrial damage, oxygen radicals and cell death in diabetes mellitus, we designed a way to investigate INS-1 cells, rat pancreatic beta-cell line, to die by treatment with alloxan which generate reactive oxygen species (ROS). Incubation of INS-1 cells with alloxan for 24 h resulted in a decrease in viability of cells as well as inhibition of glucose-stimula...

healing of skin wound is a multi-factorial and complex process. proper treatment of diabetic wounds is still a major clinical challenge. although diabetes mellitus can occur in ruminants, healing of wounds in diabetic ruminants has not yet been investigated. the aim of this study was to evaluate healing of ovine excisional diabetic wound model. eight 4-month-old iranian makoui wethers were equa...

abstract juglon is the most available flavonoide in walnut green skin and its antidiabetic effects has attracted researchers in the recent years. the aim of this study is to investigation of j.regia husk hydroalcoholic extract on biochemical blood parameters in diabetic rats. in present study, 40 male rats divided in 4 groups. 1 (normal saline) was treated by saline. 2 (control) was treated by ...

The objective of the present work was to investigate the mechanism of alloxan-induced Ca2+ release from rat liver mitochondria. Transport of Ca2+, oxidation and hydrolysis of mitochondrial pyridine nucleotides, changes in the mitochondrial membrane potential, and oxygen consumption by mitochondria were investigated. Alloxan does not inhibit the uptake of Ca2+ but stimulates the release of Ca2+ ...