Activated platelets release a multifaceted blend of growth factors that has stimulatory effects on mesenchymal cells, both in vitro and in vivo, which imply beneficial effects on wound repair and tissue regeneration. Previous studies on fibroblast cultures have revealed that more potent growth factors, with respect to cell proliferation, are released in acidic preparations of lysed platelet con...

A gene (tap) encoding a thermostable alkaline phosphatase from the thermophilic bacterium Thermus thermophilus XM was cloned and sequenced. It is 1506 bp long and encodes a protein of 501 amino acid residues with a calculated molecular mass of 54.7 kDa. Comparison of the deduced amino acid sequence with other alkaline phosphatases showed that the regions in the vicinity of the phosphorylation s...

The term "alkaliphile" is used for microorganisms that grow optimally or very well at pH values above 9 but cannot grow or grow only slowly at the near-neutral pH value of 6.5. Alkaliphiles include prokaryotes, eukaryotes, and archaea. Many different taxa are represented among the alkaliphiles, and some of these have been proposed as new taxa. Alkaliphiles can be isolated from normal environmen...

The Soret absorption band of the ferrous carbon monoxide (CO) complex of cytochrome c peroxidase exhibited a blue shift from 423.7 to 420 nm upon an increase in pH from 6.5 to 8.5. The spectral change was reversible with an isosbestic point at 422 nm. The pH dependence of this spectral change gave a sigmoidal curve fitted well to a theoretical curve of a cooperative release of two protons with ...

It is generally accepted for Escherichia coli that (i) the level of OmpC increases with increased osmolarity when cells are growing in neutral and alkaline media, whereas the level of OmpF decreases at high osmolarity, and that (ii) the two-component system composed of OmpR (regulator) and EnvZ (sensor) regulates porin expression. In this study, we found that OmpC was expressed at low osmolarit...

In this study, alkaline colloidal silica was prepared by titration of an alkaline solution such as sodium silicate with silicic acid in constant range of pH  .This constant range of pH was achieved by using concentrated soduim silicate solutions as pH adjusting solutions during titration.The sodium silicate solutions  having a variety of...

The EPR and UV-VIS spectroscopy was used to determine the pH effect on bovine liver catalase. The measurements were made in the pH range from 2 to 10. The positions, shapes and relative intensities of the UVVIS peaks were observed. Spin labelling technique was used to monitor the molecular dynamics of catalase. Treatment with acid or alkaline solutions causes spectral changes which may be due t...

The degradation behavior of cefquinome sulphate in alkaline medium at different temperatures was investigated using both first derivative spectrophotometric and HPLC methods. The drug degradation was found to be pH and temperature dependant. The pH-rate profile indicated a first order dependence of Kobs on [OH(-)] at pHs ranging between 9 and 11. Arrhenius plot obtained at pH 10 was linear betw...

The hydrolysis of 0-p-phenylazophenylphosphorothioate by Escherichia coli alkaline phosphatase was studied by the stopped-flow technique. “Burst” kinetics is observed at both acid and basic pH, suggesting that a step following thiophosphorylation is rate-limiting at all pH values. At pH 8.5, activation of a second active site on the enzyme dimer is observed in the transient phase as subsrrate c...

An alkaline protease-producing fungus was screened from potato grown soil fields of Bangalore and identified as Aspergillus terreus gr. based on morphological and microscopic features. The selected growth medium contained casein (1%) as a sole source of carbon and nitrogen. The pH of the medium was adjusted to 10.0 using sodium carbonate in order to isolate alkalophilic fungi. The best process ...