Cytosolic dynamin-related protein 1 (Drp1, also known as DNM1L) is required for both mitochondrial and peroxisomal fission. Drp1-dependent division of these organelles is facilitated by a number of adaptor proteins at mitochondrial and peroxisomal surfaces. To investigate the interplay of these adaptor proteins, we used gene-editing technology to create a suite of cell lines lacking the adaptor...

Cytoplasmic dynein is a large minus end-directed motor complex with multiple functions during cell division. The dynein complex interacts with various adaptor proteins, including the dynactin complex, thought to be critical for most dynein functions. Specific activities have been linked to several subunits and adaptors, but the function of the majority of components has remained elusive. Here, ...

Contour-shape coding is color selective (Gheorghiu & Kingdom, 2007a) and surround textures inhibit the processing of contour shapes (Gheorghiu & Kingdom, 2011; Kingdom & Prins, 2009). These two findings raise two questions: (1) is texture-surround suppression of contour shape color selective, and (2) is texture-shape processing color selective? To answer these questions, we measured the shape-f...

Geometric/Solid modelers and CAD/CAM components are moving to distributed, heterogeneous environments. Communication between such systems requires standards for data exchange. This paper describes (i) modeler independent standardized interfaces called adaptors, and (ii) feature recognition in a distributed environment. A solid modeler is augmented with an adaptor so as to provide a standard pro...

RpoS, the stationary phase/stress sigma factor of Escherichia coli, regulates a large cohort of genes important for the cell to deal with suboptimal conditions. Its level increases quickly in the cell in response to many stresses and returns to low levels when growth resumes. Increased RpoS results from increased translation and decreased RpoS degradation. Translation is positively regulated by...

Disorganized ion transport caused by hypo- or hyperfunctioning of the cystic fibrosis transmembrane conductance regulator (CFTR) can be detrimental and may result in life-threatening diseases such as cystic fibrosis or secretory diarrhea. Thus, CFTR is controlled by elaborate positive and negative regulations for an efficient homeostasis. It has been shown that expression and activity of CFTR c...

High-throughput sequencing (HTS) has become a powerful tool for the detection of and sequence characterization of microRNAs (miRNA) and other small RNAs (sRNA). Unfortunately, the use of HTS data to determine the relative quantity of different miRNAs in a sample has been shown to be inconsistent with quantitative PCR and Northern Blot results. Several recent studies have concluded that the majo...

TLR signal via Toll-IL-1R (TIR) homology domain-containing adaptor proteins. One of these adaptors, Toll-IL-1R domain-containing adaptor inducing IFN-beta-related adaptor molecule (TRAM), has been shown to be essential for TLR4 signaling in TRAM(-/-) mice and cell lines. Previously, we showed that MyD88 or Mal dominant-negative constructs did not inhibit LPS induction of cytokines in primary hu...

The integration of third-party web services can solve complex business problems and can reduce risks, costs and time-to-market. However, the task of the integrators is challenged by services that are maintained by different organisations, and that may evolve dynamically and autonomously. The impossibility of statically determining which service implementation will be bound at runtime may lead t...