We have constructed a plasmid, pPSG4, that carries only the coding sequences for polyomavirus (Py) small, middle and truncated large T-antigens. A unique HindIII site allows the introduction of foreign promoters directly in front of viral coding sequences. The simian virus 40 (SV40) early and late, adenovirus-2 (Ad-2) major late and herpes simplex virus-1 (HSV-1) thymidine kinase (TK) promoters...

BACKGROUND Familial adenomatous polyposis (FAP) is an autosomal dominant inherited disease mainly caused by mutations of the adenomatous polyposis coli (APC) gene with almost complete penetrance. These colorectal polyps are precancerous lesions that will inevitable develop into colorectal cancer at the median age of 40-year old if total proctocolectomy is not performed. So identification of APC...

Telomeres derived from the same formation event in wild type strains of Saccharomyces cerevisiae possess the same, precise TG(1-3) sequence for the most internal approximately 100 bp of the 250-350 bp TG(1-3) repeats. The conservation of this internal domain is thought to reflect the fact that telomere lengthening and shortening, and thus alteration of the precise TG(1-3) sequence, is confined ...

Human genomic DNA contains a number of diverse repetitive sequence motifs, often identified as fragile sites leading to genetic instability. Among them, expansion events occurring at triplet repeats have been extensively studied due their association with neurological disorders, including Huntington’s disease (HD). In the case HD, expanded CAG in HTT gene are thought cause onset. The is believe...

Title of Document: THE AGARASE SYSTEM OF SACCHAROPHAGUS DEGRADANS STRAIN 2-40: ANALYSIS OF THE AGARASE SYSTEM AND PROTEIN LOCALIZATION Nathan A. Ekborg, Doctor of Philosophy, 2005 Directed By: Professor Emeritus Ronald M. Weiner, Department of Cell Biology and Molecular Genetics and Professor Steven W. Hutcheson, Department of Cell Biology and Molecular Genetics Saccharophagus degradans (former...

Trichiuridae fish are economically important species and widely distributed across the nearshore to open ocean. In present study, genomic survey sequencing method was used analyze characteristics of five fish. The calculated genome size 913 Mb, 868 871 747 670 Mb for Trichiurus japonicus , nanhaiensis brevis Lepturacanthus savala Eupleurogrammus muticus respectively. average GC content ranged f...

Microsatellites or simple sequence repeats (SSRs) are very effective molecular markers in population genetics, genome mapping, taxonomic study and other large-scale studies. Variation in number of tandem repeats within microsatellite refers to simple sequence length polymorphism (SSLP); but there are a few studies that are showed SSRs replication slippage may be occurred during in vitro amplifi...

Cystic fibrosis (CF) is a life-limiting autosomal recessive disorder affecting principally respiratory and digestive system. It is caused by cystic fibrosis transmembrane conductance regulator (CFTR) gene mutation. The aim of this study was to determine the extent of repeat numbers and the degree of heterozygosity for c.3499+200TA(7_56) and D7S523 located in intron 17b and 1 cM proximal to the ...