Although engineering of transcription factors and DNA-modifying enzymes has drawn substantial attention for artificial gene regulation and genome editing, most efforts focus on affinity and specificity of the DNA-binding proteins, typically overlooking the kinetic properties of these proteins. However, a simplistic pursuit of high affinity can lead to kinetically deficient proteins that spend t...

Objective(s)Tissue engineering is an attractive science because it promises new therapeutic strategies for repairing organs that have lost functions due to damage. The purpose of this study was to evaluate induction effect of human gingival scaffold in tissue engineering for skin regeneration.Materials and MethodsTissue samples were obtained from crown-lengthening procedures and wisdom teeth re...

background: bone marrow-derived mesenchymal cellscan transdifferentiate into cardiomyocyte cells and improveheart function after transplantation. since biomaterials canimprove the cell retention in the site, cell survival and differentiation,heart tissue engineering is now being explored as anapplied solution to support cell-based therapies and increasetheir efficacy for myocardial diseases. ch...

currently autografts are gold standards for craniomaxillofacial bone regeneration. but autografts have some limitations. bone tissue engineering has been introduced to overcome these limitations. it consist three components: stem cells, growth factor, and scaffold. scaffold provides an environment for bone growth and simplifies cell adhesion, differentiation and proliferation. in this review, a...

introduction : deproteinized bovine bone mineral (bio-oss) is a xenogenic bone substitute, widely used in maxillofacial bone regeneration. the aim of this in vitro study was to investigate its influence on the growth behavior of human osteosarcoma cell line, saos-2 culture, and compare it with the physiologic dose of dexamethasone, an inductive factor for osteoblasts. materials and methods: hum...

objective: we introduce an rgd (arg-gly-asp)-containing peptide of collagen iv origin that possesses potent cell adhesion and proliferation properties. materials and methods: in this experimental study, the peptide was immobilized on an electrospun nanofibrous polycaprolactone/gelatin (pcl/gel) hybrid scaffold by a chemical bonding approach to improve cell adhesion properties of the scaffold. a...

objective: finding cell sources for cartilage tissue engineering is a critical procedure. the purpose of the present experimental study was to test the in vitro efficacy of the beta-tricalcium phosphate-alginate-gelatin (btag) scaffold to induce chondrogenic differentiation of human umbilical cord blood-derived unrestricted somatic stem cells (usscs). materials and methods: in this experimental...

objective: the incidence of heart valve disease is increasing worldwide and the number of heart valve replacements is expected to increase in the future. by mimicking the main tissue structures and properties of heart valve, tissue engineering offers new options for the replacements. applying an appropriate scaffold in fabricating tissue-engineered heart valves (tehvs) is of importance since it...

Zinc finger proteins that bind Zn(II) using a Cys2His2 coordination motif within a ββα protein fold are the most abundant DNA binding transcription factor domains in eukaryotic systems. These classic zinc fingers are typically unfolded in the apo state and spontaneously fold into their functional ββα folds upon incorporation of Zn(II). These metal-induced protein folding events obscure the free...