Some years ago it was found by one of us that creatinine reacts with 3,5-dinitrobenzoic acid in alkaline solution to give a purplish rose color, and that the reaction is sufficiently sensitive to permit its application to urine and blood filtrates.’ Particular interest is attached to a new reaction for creatinine because the Jaffe reaction with picric acid has hitherto been the only known highl...

Ever since the introduction of the calorimetric method for the determination of creatinine, potassium bichromate, in half normal solution, has been used as the standard for measuring the color obtained in the reaction of creatinine with picric acid and sodium hydroxide. That the bichromate has been serviceable for the purpose is evidenced by the fact that no other standard has ever been propose...

The aim of present study was to relate the urine bilirubin with milk likeliness Bilirubin is a substance yellow color produced during normal process body when red blood cells are broken down. It fluid in liver. helps digestion body. If it may be sign liver disordered. A test performed by using strips for this purpose. students which show positive had more attention towards drinking as compare n...

We describe the development of a handheld stamp for the production of microfluidic paper-based analytical devices. The paper surface was oxidized for allowing the covalent coupling of enzymes. The chemical modification led to improvements on the color uniformity. The relative standard deviation values for glucose and uric acid assays decreased from 40 to 10% and from 20 to 8%, respectively. The...

In 1926 Campbell and Hanna (1) published a method for the determination of fructose based upon the reduction of molybdenum in phosphoric acid solution and the reoxidation of the reduced molybdenum with potassium permanganate. This method is not entirely specific for fructose; other substances in blood and urine have some reducing action upon the molybdic acid reagent. The method will not permit...

Development of improved glucose detection has vast significance in both clinical and point of care settings. Herein, we present a novel, label-free, enzyme-free, colorimetric method of glucose detection that relies on the reduction of a gold salt precursor facilitated by physiological concentrations of glucose (1.25-50 mM). The concentration of glucose present during the reduction process resul...

Ever since the introduction of the calorimetric method for the determination of creatinine, potassium bichromate, in half normal solution, has been used as the standard for measuring the color obtained in the reaction of creatinine with picric acid and sodium hydroxide. That the bichromate has been serviceable for the purpose is evidenced by the fact that no other standard has ever been propose...

FTIPPURIC ACID 1S an important metabolic product normally present in urine in amounts determined by tile ingestion of benzyl-containing foods and medicinals (ii). Because this compound is comparatively inert chemically, identification requires isolation and the determination of physical constants after extensive purification. There has long been need for a reliable and sensitive color reaction ...

While determining the bisulfite-binding substance of urine from pyridoxine-deficient rats and their paired pyridoxine-fed controls, E. J. Wickson, a student assistant, noticed that when slightly alkaline the urine of the pyridoxine-deficient rat, had a green color, making the iodometric titration in the determination of bisulfite-binding substances impossible. The green pigment was found only i...