Cholera enterotoxin inhibits the antigen-induced. IgE-mediated release of histamine from human leukocytes and the lysis of allogeneic mastocytoma cells by splenic lymphocytes from specifically immunized mice. This effect requires a prolonged preincubation time of the toxin with the lymphocyte/leukocyte preparations: a demonstrable inhibition requires about 30 min of pre-incubation and the toxin...

This study examines the kinetics of pyrolysis and oxidation hydrochars through thermal analysis. Thermogravimetric analysis (TGA) differential scanning calorimetry (DSC) techniques were used to investigate decomposition profiles develop two distributed activation energy models (DAEM) derived from hydrothermal carbonization grape seeds produced at different temperatures (180, 220, 250 °C). Data ...

the effects of magnesium hydroxide and l-lactide dimer as additives on thermal properties and morphology of poly(l-lactide) (plla) films were studied. hence, neat plla films and films containing additives (10% w/w) were prepared in dichloromethane at room temperature via solution casting. to evaluate thermal history on polymer properties, plla films were annealed by different processes. in one ...

Two trials using a total of 1,484 Ross male broilers were conducted to study the effect of air velocities of 180 and 120 m/min versus still air (<15 m/min) on BW gain (BWG) and feed:gain from 3 to 7 wk of age. Broilers were raised in a common environment to 3 wk of age. The experimental facility was a closed sided house containing eight wind tunnel floor pens and six floor pens. There were two ...

BACKGROUND Glucagon-like peptide-1 (GLP-1) is an intestinal insulinotropic hormone that augments glucose-induced insulin secretion in patients with type 2 diabetes. It has also been proposed that a substantial component of the glucose-lowering effects of GLP-1 occurs because this hormone enhances insulin-mediated glucose disposal. However, interpretations of the studies have been controversial....

The aim of this work was to maximize the enzymatic aqueous extraction (EAE) sunflower seed oil using protease enzymes from evaluation various temperatures, pH and enzyme concentrations, a Box-Behnken experimental design. effect thermal pre-treatment seeds on free yield (FOY) quality also determined. In range adopted, lower temperature (40 °C) provided higher FOY values, as well intermediate (8....

We aimed to assess postprandial metabolic and appetite responses to a mixed-macronutrient lunch following prior addition of whey protein to a carbohydrate-rich breakfast. Ten healthy males (age: 24 ± 1 years; body mass index (BMI): 24.5 ± 0.7 kg/m²) completed three trials in a non-isocaloric, crossover design. A carbohydrate-rich breakfast (93 g carbohydrate; 1799 kJ) was consumed with (CHO + W...

Preparation of mouse genomic DNA for PCR is costly and laborious, primarily because of the difficulty in physically separating DNA from other tissue components. This level of purification is unnecessary for many PCR applications. A simple alternative, lysis in an alkaline reagent and neutralization with a suitable buffer, has been used to prepare genomic DNA from buccal swabs, whole blood, seme...

Chemical cell lysis is an interesting topic in the research to Lab-on-a-Disc (LOD) platforms on account of its perfect compatibility with the centrifugal spin column format. However, standard procedures followed in chemical cell lysis require sophisticated non-contact temperature control as well as the use of pressure resistant valves. These requirements pose a significant challenge thereby mak...

prolonged incubation. HuO lytic titers were typically lower than those for Rb in the same sera.In 91 commercial drakes complete HuO lysis occurred in 14/91 (15%), ≥ 50% lysis in 51/91 (56%), but 7 (8%) sera were negative. Heating sera reduced lytic capacity for both HuO and Rb but not to the same degree. Nearly all HuO lytic capacity was removed after 10 min exposure to 57°C while the same trea...