Cisbio offers a comprehensive line of HTRF phosphoand total protein assays to investigate cell signaling pathways. These versatile, homogeneous assays are suitable for a variety of sample types, including primary cell lines and tumor xenografts. Each HTRF assay includes a common and essential step that relies on the ability of the lysis buffer to release the protein of interest. This protein, e...

When peripheral blood lymphocytes and human red cells coated with IgG were incubated in vitro in culture medium, antibody-dependent lymphocyte-mediated lysis was observed. This lysis was markedly inhibited by the addition of purified monoclonal IgG1 (1000 microgram/ml) to the culture medium. In contrast, lysis by lymphocytes of sensitized red cells in the presence of undiluted human serum was e...

Exposure of isolated bovine neutrophils to partially purified Pasteurella haemolytica leukotoxin caused increased synthesis of leukotriene B4 (LTB4) but not thromboxane B2 (TXB2) from endogenous arachidonic acid. Synthesis of LTB4 was closely correlated with leukotoxin-induced neutrophil lysis. At low toxin concentrations, LTB4 production lagged behind leukotoxin-induced neutrophil lysis over a...

Lysis of Escherichia coli by bacteriophage xX174 is caused by the phage protein E. As protein E is devoid of enzymatic activities it has been postulated that lysis is the result of an induction of the autolytic enzymes of the host. This hypothesis was investigated by comparing the murein composition before and during lysis of either xX174 infected cells or protein E induced lysis of E. coli. Ad...

Our previous study revealed that modified MAGSi (magnetic-silica nanopartivel beads) had various sizes and shapes affected the DNA extraction process. In this study, we combined our with several kinds of lysis buffer for to analyze result. Human saliva was extracted using three MAGSi. Each them made differently. We used ATL buffer, Triton Buffer, without cell lysis. The concentration purity wer...

The 90 kDa heat shock protein, Hsp90, is an abundant molecular chaperone participating in the cytoprotection of eukaryotic cells. Here we analyzed the involvement of Hsp90 in the maintenance of cellular integrity using partial cell lysis as a measure. Inhibition of Hsp90 by geldanamycin, radicicol, cisplatin, and novobiocin induced a significant acceleration of detergent- and hypotonic shock-in...

Growing Clostridium saccharoperbutylacetonicum was lysed by sodium ion concentrations above 0.1 M (maximum effect at 0.3 M). The rate of lysis depended on the age of the culture. The most rapid lysis occurred in organisms from logarithmically growing cultures which were incubated for 3 to 5 h; organisms from stationary phase cultures were completely resistant to Na+-induced lysis. Sodium ionind...

The purpose of these studies was to determine whether nitric oxide produced by cytokine-activated murine lung vascular endothelial cells plays a role in their lytic destruction of M-5076 reticulum cell sarcoma. Vascular endothelial cells harvested from perfused lungs of mice were adapted to grow in culture. Cloned lines ascertained to be of endothelial origin were incubated in vitro with interf...

* Corresponding author. E-mail: [email protected] Throughout the long history of the bacteriophage T4 research, the phenomenon of lysis inhibition (LIN) became an exclusive point of interest of T4 physiology. An ability to control the timing of cell lysis in response to the availability of bacterial hosts in the environment is considered to contribute significantly to the prevalence of T-even pha...

Functional redundancy in genomes arises from genes with overlapping functions, allowing phenotypes to persist after gene knockouts. Evolutionary redundancy or evolvability of a genome is one step removed, in that functional redundancy is absent but the genome has the potential to evolve to restore a lost phenotype. Exploring the extent to which this recovery alters gene networks can illuminate ...