Targeting exogenous genes at milk protein loci via gene-targeting technology is an ideal strategy for producing large quantities of pharmaceutical proteins. Transcription-activator-like effector (TALE) nucleases (TALENs) are an efficient genome-editing tool. However, the off-target effects may lead to unintended gene mutations. In this study, we constructed TALENs and TALE nickases directed aga...

The TALE infill experiment is a further extension of TA-TALE detectors to observe low-energy cosmic rays down the PeV region. utilizes existing TALE-FD detectors, and newly developed "infill" surface with 100 m 200 spacing. new will be deployed at site in October-November 2022. We present design performance array hybrid mode, terms resolutions biases arrival direction, energy, X max .

introduction cellular processes can be controlled by induction or repression of gene expression by introduction of exogenous genes and rna interference (rnai), respectively. however, the use of recently-developed specific dna-binding transcription factors offer various advantages when compared to the exogenous gene introduction and rnai for up or down regulation of gene expression. sox2 gene en...

Naturally TALEs are pathogenicity factors secreted by Xanthomonas spp. into host plant cells to bind specific promoters and manipulate host transcription. Sequence specific DNA binding is conferred by the TALE repeat array. Natural TALE repeat arrays are formed of 10-30 repeats, each of which pairs with one DNA base. Collectively the repeats of a TALE array form a righthanded superhelix, enfold...

Hox genes are major regulators of embryonic development. One of their most conserved functions is to coordinate the formation of specific body structures along the anterior-posterior (AP) axis in Bilateria. This architectural role was at the basis of several morphological innovations across bilaterian evolution. In this review, we traced the origin of the Hox patterning system by considering th...

To correct a DNA mutation in the human genome for gene therapy, homology-directed repair (HDR) needs to be specific and have the lowest off-target effects to protect the human genome from deleterious mutations. Zinc finger nucleases, transcription activator-like effector nuclease (TALEN) and CRISPR-CAS9 systems have been engineered and used extensively to recognize and modify specific DNA seque...

1376 NOTICES OF THE AMS VOLUME 50, NUMBER 11 T he circle is arguably the most studied object in mathematics, yet I am here to tell the tale of circle packing, a topic which is likely to be new to most readers. These packings are configurations of circles satisfying preassigned patterns of tangency, and we will be concerned here with their creation, manipulation, and interpretation. Lest we get ...

A new Caenorhabditis elegans homeobox gene, ceh-25, is described that belongs to the TALE superclass of atypical homeodomains, which are characterized by three extra residues between helix 1 and helix 2. ORF and PCR analysis revealed a novel type of alternative splicing within the homeobox. The alternative splicing occurs such that two different homeodomains can be generated, which differ in th...

Three amino acid loop extension (TALE) homeodomain transcriptional regulators play a central role in plant and animal developmental programs. Plant KNOTTED1-like homeobox (KNOX) and animal Myeloid ecotropic viral integration site (MEIS) proteins share a TALE homeodomain and a MEINOX (MEIS-KNOX) domain, suggesting that an ancestral MEINOX-TALE protein predates the divergence of plants from fungi...

The presence of replication-competent HIV-1 -which resides mainly in resting CD4+ T cells--is a major hurdle to its eradication. While pharmacological approaches have been useful for inducing the expression of this latent population of virus, they have been unable to purge HIV-1 from all its reservoirs. Additionally, many of these strategies have been associated with adverse effects, underscori...