In this application note, we demonstrate a novel method for detecting Prostate Serum Antigen (PSA) from reduced sample volumes. The Nano eNablerTM system is used to create ultraminiaturized antibody arrays with spot sizes from 5-8 μm in diameter occupying 1/100th to 1/1000th the surface area of a conventional microarray (D=100 μm) spot [1]. This PSA level of sensitivity is several orders of mag...

We have detected sub-wavelength spot sizes in the near-field of a metal tip, which is illuminated with terahertz (THz) pulses. The THz near-field is detected by using electro-optic detection in a (100) oriented GaP crystal. Contrary to conventional electro-optic detection, which uses (110) oriented detection crystals, (100) crystals only allow the detection of THz light, polarized perpendicular...

This letter reports an optically induced cell lysis device that can selectively lyse a single cell within a group of cells, a function which cannot be performed using traditional tools. This chip-scale device was made of a photoconductive material, which can induce a nonuniform electric field at a specific position under illumination of a beam spot generating a transmembrane potential in the ce...

Chemicals and Materials All chemicals used in the experiments (KBr, Fe2(SO4)3, H2SO4, ethanol, Rhodamine B) are analytical grade or better provided by Sinopharm Co., China. All aqueous solutions are prepared with deionized water (18.2 MΩ, Milli-Q, Millipore Corp., France). ITO glass slides were washed in an ultrasonic cleaner by isopropanol and deionized water for several times and dried by blo...

Optical-resolution photoacoustic microscopy (OR-PAM) becomes a premier microscopic imaging tool in biomedicine because it provides agent-free optical absorption information in tissues. By tightly focusing light to a spot, a significantly improved lateral resolution can be achieved in OR-PAM. The focal spot size is typically determined by the numerical aperture of the used objective lens. Here, ...

The extensive lit’erature on theoretical and experimental investigations of superbroadening in small scale filaments produced by self-focussed light pulses has been reviewed by Akhmanov et al. [ 11. The interpretation of this frequency broadening in terms of moving focal spots has been reviewed more recently by Low and Shen [2]. In all of this work the diameter of the filaments, or the size of ...

A packed-bed membrane reactor in a distributor configuration is studied theoretically for the oxidative propane dehydrogenation and compared with fixed-bed reactor. Based on detailed 2D models considering two different heat mass transport scale-up including various reactor-to-particle diameter ratios (D/dP) analyzed respect to performance, transfer hot spot formation. Higher selectivities at lo...

Dear Sir, We agree with the basic strategy that Kraemer et al. (1989) outline for fitting the initial response of Limulus ventral photoreceptors to flashes of light. It now seems probable that the single photon event ("bump") arises from two separate processes, one governing the variable latency before the bump occurs and the other the amplitude and time course of the bump itself. It is reasona...

Three types of light-induced response (L-, RG-, and YRB -type S-potentials) recorded from isolated retinas of the carp (Cyprinus carpio) were identified by their spectral response and later by morphological localization of the recording sites marked with an intracellular Lucifer Yellow (LY). Horizontal cells in a given layer, generating one of the above response types, are electrically coupled ...