A novel strategy to increase protein expression yield is unintended induction of expression in complex media, called auto-induction. This method can be used to express proteins under control of the lac promoter without any need to monitor bacterial growth pattern, and addition of specific expression inducers such as Isopropyl β-D-1-thiogalactopyranoside (IPTG) at proper time. In the present stu...

background: brucellosis is still considered as one of the major zoonosis afflicting syrian health and economy. this disease is caused by members of the genus brucella which are gram-negative bacteria living facultatively within mammalian cells during infection. objectives: in this paper, a strategy was developed to introduce a new generation of binders called nanobodies (nbs) in our combat aga...

A novel strategy to increase protein expression yield is unintended induction of expression in complex media, called auto-induction. This method can be used to express proteins under control of the lac promoter without any need to monitor bacterial growth pattern, and addition of specific expression inducers such as Isopropyl β-D-1-thiogalactopyranoside (IPTG) at proper time. In the present stu...

In-line integration of sequential catalysis and continuous multi-column purification. Adapted for small compound amounts (hit-to-lead). Suitable large-scale purification (process chemistry).

Introduction Utah’s wetlands provide a constellation of recreational opportunities, from sailing to photography, fishing to birdand wildlife-watching, hunting to picnicking. Even more important are the roles wetlands play in the functioning of Utah’s ecosystems. These roles include flood control, maintenance of groundwater quality and levels, pollution control, water purification, and the provi...

zoroastrian’s purification rituals are one of the most visible aspects of zoroastrianism that root in the avesta’s texts. but it seems that these customs which were considered as a preservative of human communities from harm and evil diseases in the pre-zoroastrian period, have been changed after that period of time and their joining to the religious teachings of zoroasterian. in this paper, at...

introduction: scorpion venoms contain a variety of peptides, toxic to mammals، insects and crustaceans and are the main factors in scorpion venom toxicity (their amount being 1-3% of total venom). most of the scorpion toxins have been isolated from the venoms of scorpions in the buthidae family. the scorpion buthotus schach of this family is widely found in the western regions of iran, but no p...

to carry out the immunological experiments on the serum of multiple sclerosis (ms) patients, based on a correlation between borrelia burgdorferi infection and contracting ms autoimmune disease the outer surface protein d (ospd) of the bacterium was expressed and purified. a clone containing the ospd gene in pet11a expression vector under the control of t7 promoter was transformed to the bacteri...