Utilization of orange peels as an agroindustrial waste for production of pectin lyase (PL) [E.C.4.2.2.10] by Curvularia inaequalis (Shear) Boedijn NRRL 13884 was investigated using solid state culture (SSC). The highest level of extracellular pectin lyase was detected with this waste as an inducing substrate. The enzyme was purified using Sephadex G-100 and DEAE-Cellulose column chromatography....

Crane and Lipmann (1) have shown that arsenate uncouples oxidative phosphorylation in intact rat liver mitochondria, and that this effect can be reversed by phosphate. They postulated that arsenate competes with phosphate at the site of phosphate uptake. It has also been observed that arsenate can stimulate the respiration of intact mitochondria (1) and of submitochondrial particles’ in the abs...

Despite the number of detailed studies on arsenate adsorption onto synthetic 2-line ferrihydrite carried out during the past few decades, questions remain regarding the fate of adsorbed arsenate during phase transformation of this poorly crystalline iron oxy-hydroxide. We assessed arsenate partitioning during this transformation by aging synthetic 2-line ferrihydrite with adsorbed arsenate (at ...

Plasmid-mediated resistance to arsenate, arsenite, and antimony(III) is coordinately induced by arsenate, arsenite, antimony(III), and bismuth(III). Resistance to arsenate was recently shown [Silver, S., Budd, K., Leahy, K.M., Shaw, W.V., Hammond, D., Novick, R.P., Willsky, G.R., Malamy, M.H. & Rosenberg, H. (1981) J. Bacteriol. 146, 983-996] to be due to decreased accumulation of arsenate by t...

Kumar et al. (2015)'s review on arsenic (As) detoxification in plants and related biotechnology provides current updates about the As metabolism and recent developments in engineering As-tolerant and low-As plants. Although we agree with most of the viewpoints expressed in the review, we would like to clarify the naming and functions of ACR2, arsenate reductase, and ACR3 arsenite efflux transpo...

Discriminating against arsenate: Determination of the structure of periplasmic phosphate binding proteins at very high resolution provides the basis for understanding the high discrimination of bacteria against arsenate. The results complete our insight into earlier erroneous conclusions on the ability of certain bacteria to use arsenate instead of phosphate.

These three component inorganic ion-exchanger was synthesized under different condition. The most thermal and chemical properties of this material is prepared by intermixing solutions of sodium molybdate (0.1 M), sodium arsenate (0.1 M) and antimony(III) chloride (0.1 M) solution in different volume ratios at pH-1. The Ion-exchange capacity of all the samples was determined by column process. T...

Activated carbon was tailored with both iron and quaternary ammonium surfactants so as to concurrently remove both arsenate and perchlorate from groundwater. The iron (hydr)oxide preferentially removed the arsenate oxyanion but not perchlorate; while the quaternary ammonium preferentially removed the perchlorate oxyanion, but not the arsenate. The co-sorption of two anionic oxyanions via distin...

Desulfovibrio desulfuricans G20 grows and reduces 20 mM arsenate to arsenite in lactate-sulfate media. Sequence analysis and experimental data show that D. desulfuricans G20 has one copy of arsC and a complete arsRBCC operon in different locations within the genome. Two mutants of strain G20 with defects in arsenate resistance were generated by nitrosoguanidine mutagenesis. The arsRBCC operons ...

In this work the adsorption features of activated carbon and the magnetic properties of iron oxides were combined in a composite to produce magnetic adsorbent. Batch experiments were conducted to study the adsorption behavior of arsenate onto the synthetic magnetic adsorbent. The effects of initial solution pH, contact time, adsorbent dosage and co-existing anionic component on the adsorption o...