A fluoresceinated lectin binding assay was employed to detect carbohydrates on the sheath and cuticle of mature in vivo-derived, and immature in utero-derived Brugia pahangi microfilariae. The sheath of mature microfilariae bound concanavalin A and wheat germ agglutinin, indicating the presence of N-acetylglucosamine and glucose or mannose. In addition to binding concanavalin A and wheat germ a...

BACKGROUND Nearly all secreted proteins are glycosylated, and serum glycoproteins that exhibit disease-associated glycosylation changes have potential to be biomarkers. In rheumatoid arthritis (RA), C-reactive protein (CRP), and matrix metalloproteinase-3 (MMP-3) are widely used as serologic biomarkers, but they lack sufficient specificity or precision. We performed comparative glycosylation pr...

Five leptospiral strains were isolated from bovine kidneys during a cultural survey for pathogenic leptospires in Nigeria. Preliminary test results indicated that the five strains were identical and serologically heterologous to the other members of the Pyrogenes serogroup. Further examination of the strains by the cross-agglutinin absorption test, factor analysis and restriction endonuclease a...

approved Redacted for Privacy Ralk S. Quatrano The activity of a highly purified plant lectin, wheat germ agglutinin (WGA), has been detected in wheat embryos as young as 25 days post-anthesis. The amount of hemagglutinating activity present in young embryos increases to a maximum level at 45 days post-anthesis. No lectin activity was found in the endosperm, roots, leaves or in a wheatembryo ti...

In this study, a novel, simple and rapid hemagglutination assay by using a peanut lectin to detect a neuraminidase activity in strains of the Bacteroides fragilis group was developed. One hundred and fourteen species of the B. fragilis group isolated from children with and without diarrhea and 15 reference strains were evaluated. Neuraminidase production was determined by using the method above...

The role of glycochains of cell surface glycoproteins in the cell to collagen interaction was examined by studying the effect of lectins on the fibroblast-mediated collagen gel contraction. Lectins of Phaseolus vulgaris agglutinin (PHA), concanavalin A (ConA), lentil seed agglutinin (LCA), pea agglutinin (PSA), Ricinus communis agglutinin-60 (RCA), and wheat germ agglutinin (WGA) dose-dependent...

Ricinus communis agglutinin was subjected to various chemical treatments and the effect on its hemagglutinating and saccharide-binding properties was studied. Acetylation, succinylation and citraconylation led to a complete loss in the activity of the agglutinin, whereas reductive methylation had no effect on the activity, showing that charged amino groups were involved in the hemagglutinating ...

The distribution of lectin bindings in the testis of the smallest ruminant, lesser mouse deer (Tragulus javanicus), was studied using 12 biotinylated lectins specific for d-galactose (peanut agglutinin PNA, Ricinus communis agglutinin RCA I), N-acetyl-d-galactosamine (Dolichos biflorus agglutinin DBA, Vicia villosa agglutinin VVA, Soybean agglutinin SBA), N-acetyl-D-glucosamine and sialic acid ...