The emergence of zinc as a potent neurotoxin has prompted the development of techniques suitable for the measurement of intracellular free zinc ([Zn(2+)](i)) in cultured cells. Accordingly, a new family of Zn(2+)-sensitive fluorophores has become available. Using ionophore-induced elevations of [Zn(2+)](i) in cultured neurons, we measured [Zn(2+)](i)-induced changes in the novel dyes FuraZin-1 ...

Patch-clamp recording is a powerful approach to monitoring membrane electrical activity with high temporal resolution. However, the spatial resolution of patch-clamp recording in a distributed structure such as a neuron or a brain slice is limited by the fact that each electrode records from just one point, making recording from more than a very small number of points impractical. An approach t...

We describe the design, synthesis and fluorescent profile of a family of environment-sensitive dyes in which a dimethylamino (donor) group is conjugated to a cyanoacrylate (acceptor) unit via a cyclopenta[b]naphthalene ring system. This assembly satisfies the typical D-π-A motif of a fluorescent molecular rotor and exhibits solvatochromic and viscosity-sensitive fluorescence emission. The centr...

The DNA intercalating dye Hoechst 33342 or its close analog DCV are actively removed from cells by the multidrug resistance transporter ABCG2, a protein overexpressed in metastatic cells and somatic stem cells. In bivariate blue-red flow cytometry fluorescent plots active Hoechst or DCV efflux combined with a concentration dependent bathochromic shifts of these nuclear dyes leads to the segrega...

BACKGROUND Voltage-sensitive dyes are important tools for mapping electrical activity in the heart. However, little is known about the effects of voltage-sensitive dyes on cardiac electrophysiology. OBJECTIVE To test the hypothesis that the voltage-sensitive dye di-4-ANEPPS modulates cardiac impulse propagation. METHODS Electrical and optical mapping experiments were performed in isolated L...

Silver Halide is the most common holographic material currently used by everyone from hobbyists to professional scientists. This is because of its high sensitivity and the fact that it’s easily available commercially. Silver Halide is a salt of Silver and a Halide, which are a group of elements that include Fluorine, Chlorine, Bromine and Iodine, which are light sensitive. The first, accidental...

the photocatalytic degradation of two anionic azo dyes acid red88 (ar 88) and acid orange 7 (ao7) was investigated in aqueous solution using a tio2-containing hydrogel and uv light. the ph-sensitive hydrogel poly (styrene-alt-maleic anhydride) (psma), was synthesized and crosslinked in the presence of melamine and tio2 nanoparticles which results in entrapment of tio 2 into the melamine-grafted...

Voltage-sensitive dyes can be used to image cortical network function with millisecond temporal resolution and with a horizontal spatial resolution of approximately 50μm. This imaging technique can be combined with whole-cell patch-clamp CARL C. H. PETERSEN • Laboratory of Sensory Processing, Brain Mind Institute, SV-BMI-LSENS AAB 105, Ecole Polytechnique Federale de Lausanne, CH-1015 Lausanne,...

Two novel polyarginine labelled ruthenium polypyridyl dyes are reported, one conjugated to five, (Ru-Ahx-R5), and one to eight arginine residues, (Ru-Ahx-R8); both complexes exhibit long-lived, intense, and oxygen-sensitive luminescence; (Ru-R8) is passively, efficiently and very rapidly transported across the cell membrane into the cytoplasm without requirement for its permeablisation.

Activation and repolarization across mammalian hearts follow complex three-dimensional pathways that are governed by fiber structure, intercellular coupling, and action potentials (APs) with spatially heterogeneous properties. Voltage-sensitive dyes and imaging techniques offer new insights on how spatiotemporal heterogeneities of APs govern propagation, repolarization, and AV node conduction a...