Pretreatment of cerebral synaptic membranes with arachidonic acid (10 nM-1 microM) at 37 degrees C for 40 min caused a significant increase in [3H]clonidine binding but pretreatment at 4 degrees C did not. Scatchard analysis showed that 100 nM arachidonic acid pretreatment resulted in a significant elevation of the Bmax value of the low affinity site. PGD2, PGE2 and PGF2 alpha (10-100 microM) p...

The interaction of phytoestrogens with the most important binding sites of steroid hormones, i.e. sex hormone-binding globulin and estrogen receptors, was investigated. Relative binding affinities and association constants for 21 compounds among them isoflavones, flavones, flavonols, flavanones, chalcones and lignans were determined. The lignan nordihydroguaiaretic acid weakly displaced 17beta-...

Liposomal complexes with mucoadhesive polymers, e.g., mannosylated chitosan, are considered as prospective antituberculosis drug delivery systems. The properties of such can be critically affected by the charge and phase state liposomes. aim our work was to study interaction chitosan liposomes various compositions identify key patterns this process. We tracked titrating an increasing base-molar...

A limitation of conventional MIP in thermal and mechanical stabilities condition, improper porosity low adsorption capacity, give a reason to introduce sol-gel method molecular imprinting process recently. In this study, synthesis new molecularly imprinted polymer (SG-MIP) was studied for crystal violet (CV) selective recognition. The control non-molecularly (SG-NIP) also synthesized as referen...

Extended Hildebrand solubility approach is used to estimate the solubility of nabumetone in binary solvent systems. The solubility of nabumetone in various propylene glycol-water mixtures was analyzed in terms of solute-solvent interactions using a modified version of Hildebrand-Scatchard treatment for regular solutions. The solubility of nabumetone in the binary solvent, propylene glycol-water...

The characteristics of specific binding of the ATP-sensitive K(+) (K(ATP)) channel blocker [3H]glibenclamide to forebrain membranes (P(2) fraction, 4 degrees C) obtained from morphine-naive and -tolerant mice were evaluated. Morphine tolerance was induced by osmotic minipumps that released 45 mg/kg/day of morphine subcutaneously for 6 days. This treatment enhanced the antinociceptive ED(50) of ...

Previous analysis of fibrinogen binding to human aspirin-treated gel-filtered platelets yielded upwardly concave Scatchard plots. To ascertain whether this was due to the presence of independent heterogeneous receptor populations binding fibrinogen with different affinities, the dissociation of purified 125I-fibrinogen from ADP-treated gel-filtered platelets was evaluated as a function of recep...

Insulin interaction with four human breast cancer cell lines in tissue culture was studied with respect to specific binding to receptors, degradation, and biological respon siveness. All four lines bound and degraded 125l-labeled insulin. Binding and degradation were time, temperature, and pH dependent. Unlabeled insulin competed with 12SIlabeled insulin for binding to the breast cells with hal...