Reduced activity of the mitochondrial respiratory chain and in particular of complex I is implicated not only in the etiology of Parkinson's disease but also in other forms of parkinsonism in which striatal neurodegeneration occurs, such as progressive supranuclear palsy. The pesticide rotenone inhibits mitochondrial complex I and reproduces features of these basal ganglia neurological disorder...

The loss of dopaminergic neurons and the resultant motor impairment are hallmarks of Parkinson's disease. The SH‑SY5Y cell line is a model of dopaminergic neurons, and allows for the study of dopaminergic neuronal injury. Previous studies have revealed changes in Ether à go‑go 1 (Eag1) potassium channel expression during p53-induced SH‑SY5Y apoptosis, and the regulatory involvement of microRNA‑...

Acetate-lJ*C incorporation into long chain fatty acids by rabbit heart mitochondria was increased up to ZO-fold by citrate, isocitrate, malate, or succinate in the presence of rotenone and ATP. Rotenone blocked NADH oxidation and enabled the maintenance of a high NADH:NAD+ ratio. The rate of fatty acid synthesis was linearly related to the resulting endogenous mitochondrial NADH : NAD+ ratio. B...

Rotenone is a pesticide that has been shown to cause degeneration of dopaminergic neurons within the substantianigra and striatum. In this study, we investigated the protective effects of Apigenin on rotenone induced dopaminergic neurotoxicity. Adult male wistar albino rats were injected with rotenone (6μg/1μl in DMSO) or vehicle (1μl DMSO) into the right substantianigra pars compacta (SNpc) un...

In [ l ] low concentrations of Tinopal AN, (1,l-bis(3,N-5-dimethylbenzoxazol-2-y1)methine p-toluene sulphonate), which is present in the optical brightener Uvitex AN (Ciba-Geigy Ltd) [2], inhibited the aerobic respiration of Paracoccus denitrificans. Using inverted membrane vesicles of P. denitrifcans [I], this inhibition was shown to occur in the NADH dehydrogenase (EC 1.66.99.3) region of the...

Aging is one of the strongest risk factors for Parkinson's disease (PD). SIRT2 has been implicated in the aging process. It is pertinent to investigate the role of SIRT2 in aging-related dopaminergic neurotoxicity and to develop effective therapeutic strategies for PD through the use of aging animals. In this study, we observed that rotenone induced significant behavior abnormality and striatal...

INTRODUCTION Dopamine agonists are suggested to be more efficacious in treating Parkinson's disease (PD) as they have neuroprotective properties in addition to their receptor-related actions. AIM OF THE STUDY The present study was designed to investigate the neuroprotective effects of rotigotine, a D3/D2/D1 dopamine receptor agonist, against the two powerful complex I inhibitors, 1-methyl-4-p...

SCOPE Rotenone is a toxicant believed to contribute to the development of Parkinson's disease. METHODS AND RESULTS Using human peripheral blood lymphocytes we demonstrated that exposure to rotenone resulted in disruption of electron transport accompanied by the production of reactive oxygen species, development of apoptosis and elevation of peroxidase activity of mitochondria. Employing LC/MS...

Rotenone, an organic pesticide and potent mitochondrial complex I inhibitor, causes Parkinson-like neurodegeneration in rodents and is implicated in human Parkinson's disease. In this rapid report, rotenone induced a dose-dependent decrease in succinyl-coenzyme A (CoA) and increase in β-hydroxybutyryl-CoA in multiple human cell lines (IC(50) < 100 nM). Rotenone also inhibited [U-(13)C(6)]-gluco...