Mitochondrial biogenesis and function depend upon the interaction of mitochondrial and nuclear genomes. Forward genetic analysis of mitochondrial function presents a challenge in organisms that are obligated to respire. In the S-cytoplasmic male sterility (CMS-S) system of maize, expression of mitochondrial open reading frames (orf355-orf77) conditions collapse of developing haploid pollen. Nuc...

NADP-dependent isocitrate dehydrogenase (NADP-ICDH) is an important enzyme involved in energy metabolism. The complete coding sequence of the pepper (Capsicum annuum) NADP-ICDH gene was amplified using a reverse transcriptase PCR based on the conserved sequence information of the tomato and other Solanaceae plants and known highly homologous pepper ESTs. Nucleotide sequence analysis reveal...

Madhya Pradesh ranks first both in terms of area (25%) and production (32%) pulses India. However, it sixth after Uttar Pradesh, Bihar, Haryana, West Bengal Gujarat productivity. To realize better yield; recommended protection technologies have to be followed. Most the pigeonpea varieties been developed by traditional breeding either pedigree method or selection from landraces bi-parental matin...

A study on genetic diversity and principal component analysis (PCA) in a set of 90 fertility restorer lines rice was conducted to identify potential parents for producing high-yielding hybrids. The D2 statistics uncovered the maximum inter-cluster distance between clusters having different genotypes. genotypes PS 2, PRR 828, JR-4322-2, JR-4322-2 belonging these diversified gene pools may produc...

Cytoplasmic genic male sterility (CGMS) is a system that uses the maternal line for hybrid production, ensuring no obscurity of F1 seed purity and reducing cost production. Identification gene important plant improvement classification when using molecular marker-assisted selection (MAS) method. This study aimed to produce new (A-line) its maintainer (B-line) by transferring male-sterile from a...

Pearl millet is a climate resilient crop and one of the most widely grown millets worldwide. Heterotic hybrid development is one of the principal breeding objectives in pearl millet. In a maiden attempt to identify heterotic groups for grain yield, a total of 343 hybrid parental [maintainer (B-) and restorer (R-)] lines were genotyped with 88 polymorphic SSR markers. The SSRs generated a total ...

Considering the significance of sunflower (Helianthus annuus L.) cultivation under diverse agro-climatic conditions, present study was done to recognize assorted parental lines utilizing RAPD and ISSR markers lessen environmental effects or test blunders at Rajasthan College Agriculture, MPUA & T, Udaipur, during 2016. In study, primers produced 334 polymorphic band 160 bands. detected more...

Agrobacterium-mediated co-transformation is an efficient strategy to generate marker-free transgenic plants. In this study, the vectors pMF-2A∗ containing a synthetic cry2A∗ gene driven by maize ubiquitin promoter and pCAMBIA1301 harboring hygromycin phosphotransferase gene (hpt) were introduced into Minghui86 (Oryza sativa L. ssp. indica), an elite indica restorer line. Two independent transfo...