The carbon contents in carburized steels were investigated by electron probe microanalysis (EPMA) for a range of carbon levels in the solid solution less than 1 wt%. This article describes the difficulties encountered with the classic analytical procedure using the k ratio of X-ray intensities and the phi(rhoz) model. Here, a suitable calibration curve method is presented with emphasis on the m...

X-ray mapping (XRM) is the collection of characteristic x-rays as a function of the position of the scanning electron beam on the specimen. This analytical technique provides a high magnification image related to the distribution and relative abundance of elements within a given specimen. Consequently, XRM has become one of the most popular and useful methods of microanalysis and has been widel...

Elemental microanalysis as effected under the scanning electron microscope equipped with X -Ray spectrom eters permits the dstection in a sample of ali the elements of M endeleefs classification comprised between Berylium and Uranium. We thought it advisable to apply th is teclm ique to our research on pathogenic fungi in Man. The present paper reports our results on the mychelial phase of Spor...

The spatial resolution of X-ray microanalysis has been investigated by Monte Carlo calculatio~s, including knock-on secondary electron production. The results for AuMa in a lOOOA thin Au film at 100 keV showed an appreciable difference from the ones without knock-out events. Also calculations have been done with the Mott cross-section instead of the screened Rutherford cross-section for elastic...

The copper-containing granules in the mid-gut epithelium of larval Drosophila melanogaster were examined for acid phosphatase by combined histochemistry and energy-dispersive, X-ray microanalysis. After incubation, many of the granules were shown to contain simultaneously copper and sulphur (which are normal constituents), and lead and phosphorus (which are the detectable elements of the reacti...

The ionic composition of airway surface liquid (ASL) has been debated, and, in particular for the mouse, a wide range of values has been published. Two techniques were developed to measure the elemental composition of the ASL. X-ray microanalysis of ASL was carried out at low temperature on trachea removed from isoflurane-anesthetized animals and shock-frozen. In the second technique, dextran b...