The isolation from the rat ventral prostate of a soluble enzyme preparation producing spermidine, COZ, and methylthioadenosine from putrescine and S-adenosyl-L-methionine is described. This preparation catalyzes limited decarboxylation of S-adenosyhnethionine in the absence of any additional reactants but maximal rates of CO2 release from S-adenosyhnethionine require the presence of putrescine....

A cytosolic spermidine N-acetyltransferase has been partially purified from livers of rats treated with carbon tetrachloride or thioacetamide. This enzyme formed N’-acetylspermidine when incubated with spermidine and acetyl-coA. The enzyme was also able to acetylate spermine, norspermidine, norspermine and, at a much slower rate, 1,3-diaminopropane. Putrescine, cadaverine, homospermidine, and ...

The polyamines, putrescine, spermidine, and spermine, undergo dramatic cyclical variation in both synthesis and accumulation during the early cleavage stages of sea-urchin development. Ornithine decarboxylase activity (putrescine synthesis) in developing Strongylocentrotus purpuratus exhibits maxima at •£• and 2 h after fertilization; increases in ornithine decarboxylase activity appear to corr...

Lead and cadmium accumulation examined in shoot and leaf tissues of seedlings of mustard (Brassica juncea cv RH-30), at 7th day, treated with either putrescine (1 mM), or ammonium nitrate (10 mM) or IAA (10 microM). These were included in the nutrient medium, containing Pb or Cd (0.1 mM and 2 mM). Metal accumulation was more in shoot than in leaf tissues, which was increased manifold under sali...

BACKGROUND AND AIMS It has been suggested that putrescine acts as a growth factor in the gut, but its exact function in some aspects of cellular metabolism is still in question. The aim of the present work was to identify some functions of putrescine in small bowel metabolism. ANIMALS Rats (about 80 g), in groups of five, were given either phytohaemagglutinin- or lactalbumin-containing diets,...

To clarify the involvement of the arginine decarboxylase (ADC) pathway in the salt stress response, the polyamine titre, putrescine biosynthetic gene expression, and enzyme activities were investigated in apple [Malus sylvestris (L.) Mill. var. domestica (Borkh.) Mansf.] in vitro callus under salt stress, during recovery after stress, and when ADC was inhibited by D-arginine, an inhibitor of AD...

This study was conducted during two successive seasons 2005 and 2006 by using "Desert Red" peach cultivar (Prunus persica L.) grafted on Nemaguard rootstock. Trees were sprayed with a hand sprayer to the run off at the initiation of phase ΙΙΙ of the double sigmoid curve before the onset of the climacteric. The treatments included putrescine at 5 and 10 mM, GA3 at 200 and 400 ppm, in addition to...

Putrescine is the major source of y-aminobutyric acid (GABA) in the rat adrenal gland. Diamine oxidase, and not monoamine oxidase, is essential for GABA formation from putrescine in the adrenal gland. Aminoguanidine, a diamine oxidase inhibitor, decreases the GABA concentration in the adrenal gland by more then 70 % after 4 h, and almost to zero in 24 h. Studies using [14C]putrescine confirm th...

Marine bacteria removed two diamines, putrescine and cadaverine, from coastal seawater supplemented only with these compounds. Batch cultures of natural bacterial communities were grown in filtered seawater (0.05 mum) supplemented with 500 mug of putrescine or cadaverine per liter. Increases in bacterial cell number were counted with an epifluorescence microscope after acridine orange staining....

Polyamine (putrescine, spermidine and spermine) and agmatine uptake by the human organic cation transporter 2 (hOCT2) was studied using HEK293 cells transfected with pCMV6-XL4/hOCT2. The Km values for putrescine and spermidine were 7.50 and 6.76 mM, and the Vmax values were 4.71 and 2.34 nmol/min/mg protein, respectively. Spermine uptake by hOCT2 was not observed at pH 7.4, although it inhibite...