Objective: To improve the strain of Pleurotus mushroom by protoplast fusion technique Methods: The white rot fungus Pleurotus species was isolated from fresh fruiting body of Oyster mushroom by spore print technique and was cultured on Potato dextrose agar. The selected strain was identified based on the morphological and cultural characters on Potato dextrose agar. The protoplasts were isolate...

In this study, attempts were made to select in vitro responsible genotypes and to fuse the isolated protoplasts of Brassica carinata and Brassica napus breeding lines (BC DH Dodolla, BC DH -1, BC DH-6 and BN OP-1, BN-SL03/04), obtained from our previous experiments. Combination of three different PEG concentrations (20%, 25% or 30%) and two different treatment durations (15 and 20 min.) were te...

The genetics of Schwanniomyces alluvius, a yeast that secretes alpha-amylase, were investigated. No mating types have been detected in this haploid organism. Hybrids were produced by protoplast fusion, and these were subjected to diad analysis by using two-spored asci. Results showed that the diploidy introduced by cell fusion persists through successive spore generations. It was concluded that...

When protoplasts derived from sporulating cells of Bacillus subtilis were fused by exposure to polyethylene glycol (PEG) and fixed immediately thereafter, protoplasts with two enclosed prespores could be seen by electron microscope. The number of fusion events was greatly increased, and multiply fused protoplasts appeared, when the PEG-treated suspension was diluted in hypertonic broth and rein...

Pleurotus eous colonies regenerated on plates containing complete medium overlaid with soft agar gave 0.28% regeneration efficiency, while that of Pleurotus flabellatus was slightly less (0.24%). In PEG (polyethylene glycol) induced fusion experiments with Pleurotus eous and Pleurotus flabellatus, 0.18% of fusion frequency was achieved and four fusant colonies were recovered. The fusant No.4 co...

A preparation of two commercial enzymes was used to liberate protoplasts from 16-h-old mycelium of Phanerochaete chrysosporium. Regeneration frequencies of up to 5% were attained when the protoplasts were plated in a medium containing 10% sorbose and 3% agar. Fusion of protoplasts from different auxotrophic strains in polyethylene glycol-Ca produced heterokaryons. Separation of the heterokaryon...