OBJECTIVE To examine the role of AMP-activated protein kinase (AMPK) in the control of glucoprivic feeding by hindbrain catecholamine neurons. RESEARCH DESIGN AND METHODS Micropunched hindbrain samples were collected from control and 2-deoxy-d-glucose (2DG)-injected rats for Western blot analysis of phosphorylated (activated) AMPK (pAMPK). Samples also were collected from 2DG-injected rats pr...

Authors: Grigorios Spirakis, School of Business and Economics, University of Peloponnese, Tripolis 22100, Greece; Antonios Sarantidis, Visiting Researcher, Forecasting Laboratory, Bangor Business School, Hen Coleg, College Road, Bangor, LL57 2DG, United Kingdom; Konstantinos Nikolopoulos, Bangor University, Business School, Hen Coleg, College Road, Bangor, LL57 2DG, United Kingdom, Kostas@forta...

proteomics has emerged since 1995 for studying the total proteome of cells, tissues, or organisms at any condition. the study of proteins has a fundamental importance because they are the functional and final molecules of the gene expression process and proteomics could be a valuable technique for achieving this purpose. mass spectrometry is a complementary technique for proteomics that allows ...

NKG2D ligand surface expression is important for immune recognition of stressed and neotransformed cells. In this study, we show that surface expression of MICA/B and other NKG2D ligands is dependent on N-linked glycosylation. The inhibitor of glycolysis and N-linked glycosylation, 2-deoxy-D-glucose (2DG), potently inhibited surface expression of MICA/B after histone deacetylase inhibitor treat...

A glucose analog called 2-deoxy-D-glucose (2DG) has been successfully used to sensitize cancer cells to ROS-inducing cancer treatments such as ionizing radiation, through the inhibition of glycolysis. However, the use of 2DG can be limited by several factors such as availability, non-specific cytotoxicity, and chemoresistance under hypoxic conditions. The purpose of this study was to investigat...