Pseudomonas extremaustralis produces mainly polyhydroxybutyrate (PHB), a short chain length polyhydroxyalkanoate (sclPHA) infrequently found in Pseudomonas species. Previous studies with this strain demonstrated that PHB genes are located in a genomic island. In this work, the analysis of the genome of P. extremaustralis revealed the presence of another PHB cluster phbFPX, with high similarity ...

Polyhydroxybutyrate molecules produced by recombinant Escherichia coli were evaluated in terms of their size in solution to develop and test a hypothesis concerning the regulation of the molecular weight of PHB during in vivo polymer production. Two recombinant strains of E. coli were used to produce material for this study: DH5a/pAeT41, containing the native operon from Alcaligenes eutrophus e...

The surface of polyhydroxybutyrate (PHB) storage granules in bacteria is covered mainly by proteins referred to as phasins. The layer of phasins stabilizes the granules and prevents coalescence of separated granules in the cytoplasm and nonspecific binding of other proteins to the hydrophobic surfaces of the granules. Phasin PhaP1(Reu) is the major surface protein of PHB granules in Ralstonia e...

Polyhydroxybutyrate (PHB) is considered a potential substitute for conventional, non-biodegradable petroleum-based plastics. However, high production cost has been major drawback to the commercialization of PHB. The use low-cost lignocellulosic agricultural residues such as corn stover, together with an effective pretreatment method, can reduce costs. In this study, optimization methods stover ...

Preparation of nanoparticles is one the important ways to increase biological effectiveness materials. There are several methods prepare polyhydroxybutyrate (PHB) nanoparticles. Here, a new method used based on exposing PHB ultrasound waves under variable pH conditions. In present study, was added distilled water and adjusted 4 by HCl (1 N). The suspension exposed at 4500 kh for 25 seconds. The...

PHB degradation capabilities of seven species of Aspergillus namely Aspergillus niger, Aspergillus flavus, Aspergillus ochraceus, Aspergillus nidulans, Aspergillus terreus, Aspergillus fumigatus and Aspergillus parasiticus on the polymer produced by Rhodospseudomonas palustris KU003 were assayed . Among all the fungi investigated, the ability of A.niger to utilize the biopolymer was the highest...

The surface of polyhydroxybutyrate (PHB) storage granules in bacteria is mainly covered by proteins referred to as phasins. The layer of phasins stabilizes the granules and prevents coalescence of separated granules in the cytoplasm and unspecific binding of other proteins to the hydrophobic surfaces of the granules. Phasin PhaP1 Reu is the major surface protein of PHB granules in Ralstonia eut...

Microalgae can treat waste streams containing elevated levels of organic carbon and nitrogen. This process be economically attractive if high value products are created simultaneously from the relatively low-cost stream. Co-production two microalgal products, phycocyanin polyhydroxybutyrate (PHB), was investigated using non-axenic Arthrospira platensis MUR126 supplemental (acetate, oxalate, gly...

Polyhydroxybutyrate [P(3HB)] was produced in the transgenic tobacco harboring the genes encoding acetoacetyl-CoA reductase (PhaB) and polyhydroxyalkanoate synthase (PhaC) from Ralstonia eutropha (Cupriavidus necator) with optimized codon usage for expression in tobacco. P(3HB) contents in the transformants (0.2mg/g dry cell weight in average) harboring the codon-optimized phaB gene was twofold ...