نتایج جستجو برای: polya pcr
تعداد نتایج: 175977 فیلتر نتایج به سال:
In dynamic topic modeling, the proportional contribution of a topic to a document depends on the temporal dynamics of that topic’s overall prevalence in the corpus. We extend the Dynamic Topic Model of Blei and Lafferty (2006) by explicitly modeling document-level topic proportions with covariates and dynamic structure that includes polynomial trends and periodicity. A Markov Chain Monte Carlo ...
Introduction: The ecdysone inducible system potentially allows the study of conditional expression of the exogenous reporter genes that may be cell lethal or alter the phenotype during the selection of transfectants. The system relies on two independent transfections of plasmids named pVgRXR and pIND. Disruption of the regulatory element within the plasmid during stable integration can result i...
This paper presents a study on polyadenylation site prediction, which is a very important problem in bioinformatics and medicine, promising to give a lot of answers especially in cancer research. We describe a method, called PolyA-iEP, that we developed for predicting polyadenylation sites and we present a systematic study of the problem of recognizing mRNA 3 ́ ends which contain a polyadenylati...
Von Willebrand Factor (vWF) mRNA was identified in fractionated polyA+ RNA preparations isolated from cultured human endothelial cells. Micro-injection of specific polyA+ RNA fractions in Xenopus laevis oocytes provoked the synthesis of a vWF-like product which could be detected with an immunoradiometric assay relying on Sepharose-linked monoclonal anti-vWF IgG and different radiolabeled monocl...
The statistical properties of potential estimators of forest area for the USDA Forest Service’s Forest Inventory and Analysis (FIA) program are presented and discussed. The current FIA area estimator is compared and contrasted with a weighted mean estimator and an estimator based on the Polya posterior, in the presence of nonresponse. Estimator optimality is evaluated both theoretically and via...
Actin is a major cytoskeletal protein in eukaryotes. Recent studies suggest more diverse functional roles for this protein. Actin mRNA is known to be localized to neuronal synapses and undergoes rapid deadenylation during early developmental stages. However, its 3'-untranslated region (UTR) is not characterized and there are no experimentally determined polyadenylation (polyA) sites in actin mR...
Expressed sequence tags (ESTs) remain a dominant approach for characterizing the protein-encoding portions of various genomes. Due to inherent deficiencies, they also present serious challenges for data quality control. Before GenBank submission, EST sequences are typically screened and trimmed of vector and adapter/linker sequences, as well as polyA/T tails. Removal of these sequences presents...
The 5'and 3'end of the HIV-1 RNA genome forms a repeat (R) element that encodes a double stem-loop structure (the TAR and polyA hairpins). Phylogenetic analysis of the polyA hairpin in different human and simian immunodeficiency viruses suggests that the thermodynamic stability of the helix is fine-tuned. We demonstrated previously that mutant HIV-1 genomes with a stabilized or destabilized hai...
Data from the Encyclopedia of DNA Elements (ENCODE) project show over 9640 human genome loci classified as long noncoding RNAs (lncRNAs), yet only ~100 have been deeply characterized to determine their role in the cell. To measure the protein-coding output from these RNAs, we jointly analyzed two recent data sets produced in the ENCODE project: tandem mass spectrometry (MS/MS) data mapping expr...
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