Pyruvate kinase (PK) (EC 2.7.1.40) isozymes were assayed in normal rat liver and a series of transplantable rat hepatomas ranging widely in growth rate and degree of differentiation, with the use of gradient elution by chloride ion from columns of DEAE-cellulose. In agreement with other studies, three noninterconvertible forms were found in rat tissues: isozyme I, the major form in adult rat li...

A key component of the nitric oxide-cyclic guanosine monophosphate (cGMP) pathway in smooth muscle cells (SMC) is the type I GMP-dependent protein kinase (PK-G I). Activation of PK-G I mediates the reduction of cytoplasmic calcium concentrations and vasorelaxation. In this manuscript, we demonstrate that continuous exposure of SMC in culture to the nitrovasodilators S-nitroso-N-acetylpenicillam...

In veterinary drug development procedures, pharmacokinetic (PK) and pharmacodynamic (PD) data have generally been established in separate, parallel studies to assist in the design of dosage schedules for subsequent evaluation in clinical trials. This review introduces the concept of PK/PD modelling, an approach in which PK and PD data are generated in the same study, and used to derive numerica...

Purified heat shock transcription factor 1 (HSF1) binds to both the regulatory and catalytic components of the DNA-dependent protein kinase (DNA-PK). This observation suggests that DNA-PK may have a physiological role in the heat shock response. To investigate this possibility, we performed a comparison of cell lines that were deficient in either the Ku protein or the DNA-PK catalytic subunit v...

A sequence S is potentially Km−Pk graphical if it has a realization containing a Km −Pk as a subgraph. Let σ(Km −Pk, n) denote the smallest degree sum such that every n-term graphical sequence S with σ(S) ≥ σ(Km−Pk, n) is potentially Km−Pk graphical. In this paper, we prove that σ(Km−Pk, n) ≥ (2m−6)n−(m−3)(m−2)+2, for n ≥ m ≥ k + 1 ≥ 4. We conjecture that equality holds for n ≥ m ≥ k + 1 ≥ 4. W...

We describe the cellular and molecular biologic studies of the erythrocyte pyruvate kinase (PK) deficiency of the Amish deme in Pennsylvania. Nucleotide sequencing of the patient's PK gene showed a point mutation, CGC to CAC, corresponding to no. 1436 from the translationafinitiatkn site of the R-type PK (R-PK) mRNA, and it caused a single amino acid substitution from Arg to His at the 479th am...

The class of graphs that do not contain an induced path on k vertices, Pk-free graphs, plays a prominent role in algorithmic graph theory. This motivates the search for special structural properties of Pkfree graphs, including alternative characterizations. Let G be a connected Pk-free graph, k ≥ 4. We show that G admits a connected dominating set that induces either a Pk−2-free graph or a grap...

The core planar polarity proteins are required to specify the orientation of structures that are polarised in the plane of the epithelium. In the Drosophila melanogaster wing, the core proteins localise asymmetrically at either proximal or distal cell edges. Asymmetric localisation is thought to be biased by long-range cues, causing asymmetric complexes to become aligned with the tissue axes. C...