the present study was conducted to detect the occurrence, serotype, genotype, phylogenetic relationship and antimicrobial resistance pattern of stec from healthy goats of west bengal, india. from the 125 faecal samples collected from healthy goats, 245 isolates were identified as escherichia coli. the e. coli harbouring any gene for shiga toxins (stx1/stx2) was detected in 36 (14.7%) of the 245...

a three month-old-male red deer calf (cervus elaphus maral) was examined post mortem for the cause of death in arasbaran preserved area in east azerbaijan in september 2006. the main history of the case was the lack of colostrum intake after birth. the necropsy 6 hours after death, revealed severe general congestion especially in lungs and visceral organs (liver and jejunum). the cut surface of...

in modern medical processes, new antimicrobial drugs are being widely used in order to rapid and essential eradication of resistant bacterial strains. broad-spectrum beta-lactamases are enzymes originated from mutations in the common plasmid genes mediators for beta-lactamases such as tem-1, tem-2, and shv-1 are transferred among bacterial species. the genes coding the production of esbl are of...

AIM The purpose of this study was to determine the virulence genes and serotype of Shiga toxin producing Escherichia coli (STEC) strains isolated from animals and birds. MATERIALS AND METHODS A total of 226 different samples viz., fecal, intestinal content, rectal swab and heart blood were collected from different clinically affected/healthy animals and birds and were streaked on McConkeys' l...

Crystalline L-arginine deiminase of Pseudomonas putida was prepared by the following steps: sonic disruption, ammonium sulfate fractionation, protamine sulfate treatment, DEAE-cellulose column chromatography, and L-arginine-Sepharose 6B chromatography followed by crystallization. This procedure yields a crystalline pure enzyme with a 45% recovery of the activity in crude cell-free extracts. The...

The challenges we face in our economies and societies in our divided unsustainable world are perhaps greater than at any other time. These challenges have arisen because of how we have been trained to think, plan and act as individuals and how we have applied this training to the way we organise and govern ourselves. We have thought, planned, organised, governed and acted as though our world is...

conclusion our results showed that obtained protein patterns of the isolated strains were more reliable than serotyping results for typing purposes. our findings indicated that pap adhesion–encoding operon has an important role in the development and severity of uti. many cases of serious urogenital diseases are caused by a limited number of uropathogenic e.coli strains that generally possess s...