Now, as before, transmission electron microscopy (TEM) is a widely used technique for the determination of virions size. In some studies, dynamic light scattering (DLS) has also been applied for this purpose. Data obtained by different authors and using different methods could vary significantly. The process of TEM sample preparation involves drying on the substrate, which can cause virions to ...

BACKGROUND Dynamic actin assembly is required for diverse cellular processes and often involves activation of Arp2/3 complex. Cortactin and N-WASp activate Arp2/3 complex, alone or in concert. Both cortactin and N-WASp contain an acidic (A) domain that is required for Arp2/3 complex binding. RESULTS We investigated how cortactin and the constitutively active VCA domain of N-WASp interact with...

Ethylenediaminetetraacetic acid (EDTA), nitrilotriacetic acid (NTA), and other synthetic polycarboxylic acids have been shown to possess substantial capacity as washing agents of heavy metal-polluted soils, but they are environmentally problematic. Therefore, a sample of natural soluble humic substances (HS) was tested as a possible substitute. The efficiency of HS to extract cadmium (Cd), copp...

An antibody-free diagnostic reagent has been developed based on the aggregation-induced colorimetric change of Ni(II)NTA-functionalized colloidal gold and silver nanoparticles. This diagnostic strategy utilizes the high binding affinity of histidine-rich proteins with Ni(II)NTA to capture and cross-link the histidine-rich protein mimics with the silver and gold nanoparticles. In model studies, ...

objective(s): microarrays are potential analyzing tools for genomics and proteomics researches, which is in needed of suitable substrate for coating and also hybridization of biomolecules.   materials and methods: in this research, a thin film of oxidized agarose was prepared on the glass slides which previously coated with poly-l-lysine (pll). some of the aldehyde groups of the activated agaro...

the aim of this work was to study the ability of two biodegradable chelating agents, nitrilotriaceticacid (nta) and ethylenediiminopropanedioic acid (eddm) to complex metals, in the treatment of the pulp,prior to a totally chlorine free (tcf) bleaching process, and compare with the ethylenediaminetetraacetic acid(edta) performance. firstly, speciation efficiency index (sei) was calculated by co...

Structural and mechanistic characterization of proteins by fluorescence resonance energy transfer (FRET)1,2 requires the ability to incorporate fluorescent probes at specific, defined sites.2 For proteins that do not contain cysteine residues, site-specific fluorescent labeling can be accomplished by use of site-directed mutagenesis to introduce a cysteine residue at the site of interest, follo...

This paper describes an approach to synthesize a tightly adhered Ta3N5 nanotube array (NTA) photoanode with enhanced electron conductivity between the Ta3N5 layer and the substrate via a two-step anodization method. The obtained tightly adhered Ta3N5 NTA photoanode exhibits excellent photoelectrochemical properties with an optimal photocurrent up to 5.3 mA cm(-2) at 1.6 V vs. the reversible hyd...