Plantlets grown in conventional tissue culture systems usually encounter physiological and anatomical abnormalities including inability to photosynthesize, low chlorophyll content, open stomata, lack of a cuticle layer in the leaf, abnormal xylem parenchyma etc. Photoautotrophic and photomixotrophic systems could diminish these problems. The purpose of this study was to increase the chlorophyll...

The interactions of Jurkat cells with cisplatin, cis-[Pt(15NH3)2Cl2]1, are studied using 1H-15N heteronuclear single quantum coherence (HSQC) NMR and inductively coupled plasma mass spectrometry. We show that Jurkat cells in culture rapidly modify the monocarbonato complex cis-[Pt(15NH3)2(CO3)Cl]- (4), a cisplatin species that forms in culture media and probably also in blood. Analysis of the H...

The in vitro growth and morphogenesis of mouse embryos from early primitive-streak stage to early-somite stage is described. The embryo culture method employs a static culture system, a conventional chemically defined medium (Dulbecco's modified Eagle's medium), supplemented with additional glucose, glutamine and suitably prepared serum. The method of serum preparation is important for successf...

INTRODUCTION: Tuberculosis is one of the major health problems particularly in developing countries. For definitive diagnosis of pulmonary tuberculosis identification of tubercle bacilli in sputum by microscopy and culture is essential. For decontamination and concentration of sputum, the commonly used method in the laboratory is Modified Petroff’s method but the Hypertonic saline–sodium hydrox...

We analyzed DNA damage, mitotic activity and polyploidization in Crepis capillaris callus cells during shortand long-term in vitro culture, and the influence of plant growth regulators on these processes. Changes in the concentration of growth regulators altered the stability of callus. The level of DNA damage was highly dependent on the growth regulator composition of the medium. Cytokinin at ...

plantlets grown in conventional tissue culture systems usually encounter physiological and anatomical abnormalities including inability to photosynthesize, low chlorophyll content, open stomata, lack of a cuticle layer in the leaf, abnormal xylem parenchyma etc. photoautotrophic and photomixotrophic systems could diminish these problems. the purpose of this study was to increase the chlorophyll...

The initial growth of mycobacteria from 49 samples of cattle and buffalo organs collected in commercial slaughterhouses was compared between modified Middlebrook 7H11 thin layer microcolony culture and Stonebrink medium used in the isolation of Mycobacterium bovis. Aliquots were decontaminated by Petroff's method, processed and cultured in both media. The identity of the acid-fast bacilli stain...