In this issue of Cell, use live-fluorescence microscopy to monitor individual genomic loci as they replicate in budding yeast. They confirm that DNA is recruited to replication factories and show that sister replication forks initiated from the same origin are held together within a single replication factory.

Rapid advances in live-cell imaging have now enabled direct observation of the transcription of single nascent mRNA molecules from an endogenous yeast gene. A novel quantitative fluctuation analysis of fluorescently labeled mRNA revealed the kinetics of transcription initiation and the dynamics of elongation and termination.

fuller, in 1989, described probiotic microorganisms as “a live microbial feed supplement,which beneficially affects the host animal, by improving its intestinal microbial balance”.saccharomyces cerevisiae var. boulardii (s.boulardii) is an accurate probiotic yeast idol.the detection and budding of s.boulardiiis firmly related to the impression of healthinessto promote microorganisms from foodst...

endophytes are important resources of bioactive compounds and due to their potential in theproduction of secondary metabolites, they are regarded as a potential reservoir ofbiotechnological applications. in this study, the bioactivity of bacteria and yeasts endophytesresiding in thymus sp. was evaluated. during april to october 2011, symptomless and healthytissues of thymus sp. were collected. ...

Fluorescence microscopy of the localization and the spatial and temporal dynamics of specifically labelled proteins is an indispensable tool in cell biology. Besides fluorescent proteins as tags, tag-mediated labelling utilizing self-labelling proteins as the SNAP-, CLIP-, or the Halo-tag are widely used, flexible labelling systems relying on exogenously supplied fluorophores. Unfortunately, la...

Submitted for the MAR05 Meeting of The American Physical Society Dielectric Properties of Live Yeast Cells Expressed with the Motor Protein Prestin JOHN MILLER, DHARMAKEERTHI NAWARATHNA, DAVID WARMFLASH, University of Houston, FRED PEREIRA, WILLIAM BROWNELL, Baylor College of Medicine — We report on the linear and nonlinear dielectric properties of budding yeast (S. cerevisiae) cells, one strai...

Super-resolution fluorescence microscopy has emerged as a powerful tool for studying molecular organization, but mostly in fixed cells. New work using high-speed fluorescence photoactivation localization microscopy now reveals the organization of cytokinesis nodes and contractile rings in live fission yeast cells.

The effects of adding yeast culture (Saccharomyces cerevisiae, 5 x 10(9) live organisms/g of growth medium) at 10 g/steer daily to three diets consisting of 75% alfalfa silage and 25% barley, 96% corn silage and 4.0% soybean meal, or 75% dry-rolled barley and 25% alfalfa hay on performance of growing and finishing steers and carcass characteristics, feed digestibility, and degradability in the ...

A total of 27 sows (Line 241; DNA Genetics) were used in a study to evaluate the effect feeding live yeast and extracts lactating on antimicrobial susceptibilities fecal E. coli. Sows blocked by BW parity d 110 gestation allotted 1 2 dietary treatments. Dietary treatments consisted standard corn-soybean meal lactation diet or that contained yeast-based pre- probiotics (0.10% Actisaf Sc 47 HR+ 0...

Candida albicans causes candidiasis, secretes farnesol, and switches from yeast to hyphae to escape from macrophages after phagocytosis. However, before escape, macrophages may respond to C. albicans' pathogen-associated molecular patterns (PAMPs) through toll-like receptor 2 (TLR2) and dectin-1 receptors by expressing cytokines involved in adaptive immunity, inflammation, and immune regulation...