George L. Petrikkos, Associate Professor, 1st Department of Propedeutic Medicine, Athens University School of Medicine, Laiko, General Hospital, GR-115 27 Athens (Greece) From the previous very interesting presentations the following main points can be outlined: Dr. Höffken noted that the isolation of the agent responsible for respiratory tract infections (RTIs) is desirable, but it is difficul...

Klebsiella species normally produce a chromosomally encoded group 2b, or molecular class A, -lactamase. In most strains of Klebsiella pneumoniae the enzyme has an isoelectric point (pI) of 7.6, identical to that of the plasmidencoded -lactamases SHV-1 and SHV-2, and is assumed to be SHV-1. This -lactamase is transposon-encoded and it has been postulated that it might have arisen as a klebsiella...

The Met69-->Ile mutant of the OHIO-1 beta-lactamase, an SHV-family enzyme, is resistant to inactivation by beta-lactamase inhibitors. Analysis of purified Met69-->Ile enzyme reveals that its isoelectric point (pI 7.0) and CD spectrum are identical with those of the OHIO-1 enzyme. Levels of beta-lactamase expression in Escherichia coli as determined by immunoblotting are similar for OHIO-1 and M...

New antibiotic regimens are needed for the treatment of multidrug-resistant tuberculosis. Mycobacterium tuberculosis has a thick peptidoglycan layer, and the penicillin-binding proteins involved in its biosynthesis are inhibited by clinically relevant concentrations of beta-lactam antibiotics. beta-Lactamase production appears to be the major mechanism by which M. tuberculosis expresses beta-la...

Antibiotic resistance being called as the ‘ticking time bomb’ of the 21 st century, as the antibiotic channel is losing sheen and is on a standstill due to lack of initiatives for new antibiotic development, and other novel solutions are required for its revival. Penicillin, the oldest among the antibiotics, obtained from the Penicillium mold, was one of the first antibiotics used to treat infe...

In Pseudomonas aeruginosa, resistance to cefclidin is usually associated with resistance to another third-generation cephalosporin, ceftazidime. In this study we analysed 22 isolates of P. aeruginosa, collected at Showa University Fujigaoka Hospital between 1992 and 1993, which were resistant to cefclidin but susceptible to ceftazidime. All polymerase chain reaction (PCR) products amplified by ...

Typhoid fever caused by the bacteria Salmonella typhi gained resistance through multidrug-resistant S. strains. One of reasons behind β-lactam antibiotic is -lactamase. L, D-Transpeptidases responsible for typhoid as it involved in toxin release that results humans. A molecular modeling study these targeted proteins was carried out various methods, such homology modeling, active site prediction...

A total of 113 blood culture isolates of Klebsiella pneumoniae from 10 hospitals in northern Taiwan were studied for SHV and TEM beta-lactamase production. bla(SHV) was amplified from all isolates by PCR. TEM-type resistance, was found in 32 of the isolates and was of the TEM-1 type in all isolates. SHV-1, -2, -5, -11, and -12 and two novel enzymes were identified. These novel enzymes were desi...

Klebsiella quasipneumoniae subsp. similipneumoniae strain ATCC 700603, formerly known as K. pneumoniae K6, is known for producing extended-spectrum β-lactamase (ESBL) enzymes that can hydrolyze oxyimino-β-lactams, resulting in resistance to these drugs. We herein report the complete genome of strain ATCC 700603 and show that the ESBL genes are plasmid-encoded.

-lactamases are a major cause of resistance to the -lactam antibiotics. Many classes of -lactamases have been described depending on substrate specificity and susceptibility to inhibitors [1]. Amp C -lactamases are able to hydrolyse all penicillins and cephalosporins and are not inhibited by clavulanic acid. Bacteria that produce these enzymes are resistant to all penicillins including lac...