The liver plays a key role in maintaining physiological homeostasis and hepatocytes are largely responsible for this. The use of isolated primary hepatocytes has become an essential tool for the study of nutrient physiology, xenobiotic metabolism and several liver pathologies. Since hepatocytes are removed from their normal environment, the isolation procedure and in vitro culture of primary he...

Very recently, ToMITA and coworkers' 2) have reported the fermentation, isolation and structure elucidation of gilvocarcins V (1) and M (2). They note that I was previously described under the name of toromycin by HORII et al.3) although the structure of toromycin was not elucidated. Recently we have isolated 1 and 2 and a related compound gilvocarcin E (3) from a culture of Streptomyces anandi...

Dengue virus isolation and serotyping was carried out in 105 clinically-suspected cases of dengue fever by the mosquito inoculation and cell culture techniques to find a suitable virus isolation technique for a resource-constrained country like Bangladesh and to identify the serotypes responsible for the dengue outbreak in 2000. Virus was identified by direct fluorescent antibody technique (DFA...

BACKGROUND The isolation of non-tuberculous mycobacteria (NTM) has been increasing in South Korea. To date, however, the cause of this increase has not been determined, and it remains unclear whether the use of liquid media has contributed to this increase. The aim of this study was to evaluate the factors associated with NTM isolation and the impact of liquid media on NTM culture. METHODS My...

The use of 10% oxgall and bile broth medium, both supplemented with freshly prepared 100 u/ml streptokinase, for isolating Salmonella typhi by clot culture technique was evaluated and compared against whole blood culture systems (3 ml blood in 9 ml media and 8 ml blood in 72 ml media). These gave a 1:4 and 1:10 blood to medium ratio, respectively. Clot cultures in 10% oxgall (CLOX) gave a 57% p...