Whether hydrolytic or synthetic, glycosyl transfer is, simply in terms of quantity, one of the most important biological reactions on earth. The formation and cleavage of glycosidic bonds are catalysed by glycosyltransferases and glycoside hydrolases which are crucial for a number of biological pathways (biosynthesis and degradation of structural and storage polysaccharides, cellular signalling...

Inhibitors are important elements of regulation of enzyme activity. They can be named ubiquitous components in living organisms (Fan & Wu, 2005). There is a number of inhibitors, such as protein and nonprotein. Among all inhibitors, the most significant are inhibitors of hydrolytic enzymes. Hydrolases are enzymes that catalyze the hydrolysis of chemical bonds. For example, proteolytic enzymes (...

The best-characterized members of the M23 family are glycyl-glycine hydrolases, such as lysostaphin (Lss) from Staphylococcus simulans or LytM aureus. Recently, enzymes with broad specificities were reported, EnpACD Enterococcus faecalis, that cleaves D,L peptide bond between stem and a cross-bridge. Previously, activity was demonstrated only on isolated peptidoglycan fragments. Herein we repor...