Biochemical estimation of NADH concentration is a useful method for monitoring cellular metabolism, because the NADH/NAD+ reduction-oxidation pair is crucial for electron transfer in the mitochondrial electron chain. In this article, we present a novel method for deriving functional maps of intracellular reduction-oxidation ratio in vivo via measurement of the fluorescence lifetimes and the rat...

Sensing ion or ligand concentrations, physico-chemical conditions, and molecular dimerization or conformation change is possible by assays involving fluorescent lifetime imaging. The inherent low throughput of imaging impedes rigorous statistical data analysis on large cell numbers. We address this limitation by developing a fluorescence lifetime-measuring flow cytometer for fast fluorescence l...

The design of various engineering structures, such as buildings, infrastructure, aircraft, ships, as well as microelectronic components and medical implants, must ensure an extremely low probability of failure during their service lifetime. Since such a low probability is beyond the means of histogram testing, we must rely on some physically based probabilistic model for the statistics of struc...

We investigate the recombination activity of a bulk silicon defect limiting the lifetime of high quality n-type float-zone (FZ) silicon wafers. By isochronal annealing between 200 and 1100 ̊C, a defect was found to become activated upon annealing at 450–700 ̊C, causing an order of magnitude reduction in the bulk lifetime. From photoluminescence imaging, it was evident that recombination active ...

We compared lifetime and population energy budgets of the extraordinary long-lived ocean quahog Arctica islandica from 6 different sites—the Norwegian coast, Kattegat, Kiel Bay, White Sea, German Bight, and off northeast Iceland—covering a temperature and salinity gradient of 4–10 C (annualmean) and 25–34, respectively. Based on vonBertalanffy growth models and size–mass relationships, we compu...

The world's forests play a pivotal role in the mitigation of global climate change. By photosynthesis they remove CO2 from the atmosphere and store carbon in their biomass. While old trees are generally acknowledged for a long carbon residence time, there is no consensus on their contribution to carbon accumulation due to a lack of long-term individual tree data. Tree ring analyses, which use a...

This paper proposes a new measure for network performance evaluation called topology lifetime. The measure provides insight into which one of a set of topologies is likely to last the longest before more capacity must be installed. The lifetime measure is not single valued, but considers growth as a function of a set of demand shifts (perturbation). One network may be better able to support a u...