Tobacco smoking is regarded as one of the most significant risk factors in the development and progression of periodontal disease. Studies have shown an altered host antibody response to antigenic challenge in smokers with periodontitis. The study was undertaken to estimate the gingival crevicular fluid (GCF) immunoglobulin G,A,M (IgA, IgG and IgM) levels in smokers and non smoker with periodon...

Tannerella forsythia is a poorly studied pathogen despite being one of the main causes of periodontitis, which is an inflammatory disease of the supporting structures of the teeth. We found that despite being recognized by all complement pathways, T. forsythia is resistant to killing by human complement, which is present at up to 70% of serum concentration in gingival crevicular fluid. Incubati...

OBJECTIVE To compare the periodontal health of maxillary and mandibular anterior teeth retained with two types of fixed retainers. MATERIALS AND METHODS A fixed straight retainer (SR) group had 39 subjects, and a fixed wave retainer (WR) group had 35 subjects. Subjects were between the ages of 13 and 22 years and had been in fixed retention for 2 to 4 years. Pocket probing depths, bleeding on...

BACKGROUND Cytokines are suggested to play a role in periodontitis. OBJECTIVES To determine and compare the levels of Interleukin-1 beta (IL-1β) and Tumor necrosis factor alpha (TNF-α) in gingival crevicular fluid (GCF) samples amongst healthy individuals and those with chronic periodontitis. Further to compare the GCF cytokine levels in three genotype classes defined by the respective gene p...

Aim To evaluate the effectiveness on tissue response of the new nutritional supplement made of oligomeric proanthocyanidins in induced gingivitis after 21 days of use. Material and Methods A prospective, double-blind, randomized, controlled clinical trial was carried out on 20 patients; it is divided into an experimental group and a control group after fulfilling the selection criteria. Patie...

OBJECTIVES To investigate the relation between occlusal loading and peri-implant clinical parameters (probing depth, bleeding on probing, gingival retraction, width of keratinized mucosa, and crevicular fluid volume) in patients with implant-supported complete fixed prostheses in both arches. MATERIAL AND METHODS This clinical study took place at the University of Valencia (Spain) dental clin...

A high-performance hquid chromatographic assay for total hydroxyproline in serum and gingival crevicular fiuid has been developed. The Clq component of the first complement factor was precipitated out of the samples with a 0.02 M sodium acetate solution. Ahquots of the supernatants were dried, vapor hydrolyzed with 6 M hydrochloric acid at 105°C for 24 hours and derivatized with phenylisothiocy...

Introduction: Gingival crevicular fluid (GCF) has been widely investigated as a potential source of biomarkers for an individual’s oral and general health information. Various molecules presented in GCF were reported as potential biomarkers for orthodontic tooth movement (OTM). MicroRNAs (miRNAs) are small non-coding RNAs that are involved in post-transcriptional gene regulation. Recently, secr...