Fas ligand (FasL) produced by activated T cells mediates autocrine-induced apoptosis to limit T cell expansion. To investigate the regulation of FasL activity, Jurkat cells were stably transfected with a 2.3-kb fragment of human FasL promoter that controlled the expression of a GFP reporter gene. Two populations of Jurkat cells with different levels of GFP expression were obtained. One populati...

The phytochrome (phy) family of plant photoreceptors controls various aspects of photomorphogenesis. Overexpression of rice phyA-green fluorescent protein (GFP) and tobacco phyB-GFP fusion proteins in tobacco results in functional photoreceptors. phyA-GFP and phyB-GFP are localized in the cytosol of dark-adapted plants. In our experiments, red light treatment led to nuclear translocation of phy...

The ability to use GFP-tagged proteins to perform correlative light and electron microscopy has been a desirable but elusive method. The high quantum yield of GFP that makes it excellent for fluorescence imaging also makes it extremely inefficient for production of the reactive oxygen needed to trigger formation of an electron-dense precipitate for electron microscopy. This roadblock has finall...

The turnip crinkle virus-based vector TCV-GFP Delta CP had been devised previously to study cell-to-cell and long-distance spread of virus-induced RNA silencing. TCV-GFP Delta CP, which had been constructed by replacing the coat protein (CP) gene with a green fluorescent protein (GFP) coding sequence, was able to induce RNA silencing in single epidermal cells, from which RNA silencing spread fr...

....................................................... 3 Drivers for Ethernet in the metropolitan network ..................... 4 Introduction to GFP ..................................... 5 Frame mapped GFP .......................................... 5 Transparent mapped GFP ................................. 5 Structure of GFP frame .............................. 7 GFP encapsulation techniques ...

Abstract Cell health and stress readouts are critical indicators of altered or impaired function in normal diseased states cells, work has been underway to develop improved small molecule sensor dyes compatible with traditional imaging High Content Analysis (HCA) interrogation apoptotic mitochondrial pathways. The CellEvent™ Caspase Green dye effectively reports caspase activation, but suffers ...

BACKGROUND AND AIM Green fluorescent protein (GFP) is a widely used molecular tag to trace transplanted cells in rodent liver injury models. The differing results from various previously reported studies using GFP could be attributed to the immunogenicity of GFP. METHODS Hepatocytes were obtained from GFP-expressing transgenic (Tg) Lewis rats and were transplanted into the livers of wild-type...

Background Chimeric antigen receptor therapy – although very efficacious in B cell malignancies is facing many challenges which limit its success solid tumors, e.g. on-target off-tumor toxicities, heterogeneity, lack of T migration into tumors and an immunosuppressive tumor microenvironment. To better control effects address heterogeneity we developed a modular approach where equipped cells wit...