ABSTRACT- In order to produce transgenic rice lines lacking selectable marker gene, biolistic co-transformation technique using gold nanoparticles was adopted. In the first step, the efficiency of different sizes of gold particles was evaluated. The results showed that the efficiency of the nanoparticles in the transformation was comparable to that of the micro particles. Subsequently, two sepa...

The aim of the present study was to assess transcription Listeria monocytogenes key virulence genes, namely sigB, prfA, hly, plcA, plcB, inlA, inlB, inlC and inlJ during subsistence on surface tomato, cucumber carrot stored at 4, 10 30 °C for 0, 0.5, 6 24 h. Gene relative assessed through reverse transcription-quantitative polymerase chain reaction (RT-qPCR). results obtained, indicated that pl...

Relative lifetimes of inherent double stranded DNA openings with lengths up to ten base pairs are presented for different gene promoters and corresponding mutants that either increase or decrease transcriptional activity, in the framework Peyrard-Bishop-Dauxois model. Extensive microcanonical simulations used, energies physiological temperature. The bubble lifetime profiles along sequences demo...

the sucrose non-fermenting 1-related protein kinase 2 (snrk2) family members are plant unique serine/threonine kinases which play a key role in cellular signaling in response to abiotic stresses. the three snrk2 members including srk2d, srk2i and srk2e are known to phosphorylate major abscisic acid (aba) responsive transcription factors, abf2 and abf4, involved in an aba-dependent stress signal...

t cells have been identified as key players in the pathogenesis of type 1 diabetes. however, the exact role of t-cell subpopulations in this pathway is presently unknown. the purpose of this study was to assess the expression pattern of two lineage-specifying transcription factors gata - 3 and t - bet , which are important in t helper type 1 (th1) and th2 cell development , respectively. gene e...

The aims of this study were to compare the promoter sequence of the mannose-binding lectin (cMBL) gene in Iranian native and commercial chicken strains; as well as to compare the cMBL gene expression in crossbred and inbred chickens. In total 79 native (Western Azerbaijan native fowls, WANF) and 49 commercial (Arian Commercial Strain, ACS) birds were reared as parents under same management prac...

increased fetal hemoglobin (hbf) in b-globin gene disorders ameliorates the clinical symptoms of the underlying disease. 5-azacytidine, butyrate and hydroxyurea, have been shown to activate g-globin gene expression. it has also been found that hematopoietic growth factors can influence expression of g-globin in erythroid cultures and in animal models. this study was designed to evaluate the in ...