The hypoxia-inducible factor (HIF) prolyl hydroxylase PHD3 regulates cellular responses to hypoxia. In normoxia the expression of PHD3 is low and it occurs in cytosolic aggregates. SQSTM1/p62 (p62) recruits proteins into cytosolic aggregates, regulates metabolism and protein degradation and is downregulated by hypoxia. Here we show that p62 determines the localization, expression and activity o...

Olmesartan medoxomil (OLME) belongs to a group of angiotensin II receptor blockers used as an antihypertensive agent and is currently being used for prevention of hypertension. This paper describes the validation and development of stability indicating RP-HPLC method for the determination of OLME in the presence of its degradation products generated from forced degradation study and characteriz...

The quantitative analysis of tizanidine hydrochloride in both pharmaceutical dosage form and bulk medication has been developed validated using an easy, affordable, quick, unique isocratic HPLC approach. Tizanidine was separated isocratically Waters symmetry C18 ODS as the stationary phase (250×4.6 mm, 5μm particle size), a flow rate 1.0 ml/min, UV detector to track eluate at 230 nm. drug its d...

Two sensitive, precise and cost-effective UV-spectrophotometric methods are described for the determination of terbinafine hydrochloride (TFH) in bulk drug and tablets. The methods are based on the measurement of absorbance of TFH in 0.1M HCl at 222 nm in method A and in 0.1M acetic acid at 282 nm in method B. As per the International Conference on harmonization ICH guidelines, the methods were...