A fluorescent antibody reagent (termed anti-LPS conjugate) was prepared from sera obtained from hens immunized with gonococcal R-type lipopolysaccharide. The reagent was absorbed with Formalin-treated cells of Neisseria meningitidis. The anti-LPS conjugate gave uniform brilliant staining of Neisseria gonorrhoeae with little background fluorescence, thus making interpretation and reading of fluo...

Human liver sections were stained with anti-human serum albumin and/or anti-human fibrin monomer fluorescent conjugates. Approximately 10 per cent of the hepatic cells stained specifically for human serum albumin,1 per cent for fibrinogen, and 0.1 per cent for both. Approximately 18 per cent of the Kupffer cells stained specifically for human serum albumin and 33 per cent for fibrinogen. Staini...

Fluorescent antibody staining experiments with both isolated myofibrils and muscle fibers grown in culture show that AMP deaminase is bound to the myofibril in the A band. The strongest staining occurs at each end of the A band. The approximate width of the fluorescent stripes and their relation to the A band remains constant as a function of sarcomere length. Removal of enzyme from the myofibr...

Nucleic acids that have been subjected to electrophoresis through agarose gels may be detected by staining and visualized by illumination with 300-nm UV light. Methods for staining and visualization of DNA using either ethidium bromide or SYBR Gold are described here. The most convenient and commonly used method to visualize DNA in agarose gels is staining with the fluorescent dye ethidium brom...

amorphophallus campanulatus (roxb.) blume belonging to the family of araceae, is a perennial herb commonly known as elephant foot yam. its tuber has been traditionally used for the treatment of liver diseases, abdominal tumors, piles. the aim of the present study was to evaluate the dose-dependent cytotoxic and apoptosis inducing effects of the sub fractions of amorphophallus campanulatus tuber...

The conventional sputum microscopy is the standard method for detection of pulmonary tuberculosis is suspected cases. ZN staining is specific but less sensitive compared to fluorescent microscopy. The objective of the present study was to evaluate the sensitivity and specificity of ZN microscopy compared with conventional Fluorescent microscopy for examination of sputum in suspected pulmonary t...

Fluorescent probes are extensively used for detection and monitoring of live cells, in particular, due to its ability distinguish different cell types. Therefore, gating‐oriented live‐cell distinction (GOLD) mechanism describes how the unique composition membrane transporters controls influx efflux dyes create a distinct staining pathway. Herein, series techniques detailed elucidation known unk...

p-nonylphenol (p-np) as a non ionic surfactant is used in many industries such as pesticides, cosmetics,detergents and so on. our previous study showed that the p-np causes reduction in viability of the rats bonemarrow mesenchymal stem cells (mscs). the aim of the present study was to investigate the mechanism of the cell death due to p-np exposure. the cells, after 3rd passage were treated wit...