Our objective was to investigate the miRNA profile of embryonic tissues in ectopic pregnancies (EPs) and controlled abortions (voluntary termination of pregnancy; VTOP). Twenty-three patients suffering from tubal EP and twenty-nine patients with a normal ongoing pregnancy scheduled for a VTOP were recruited. Embryonic tissue samples were analyzed by miRNA microarray and further validated by rea...

Embryonic Mortality is the major cause of reproductive and economic loss in cattle Buffaloes. more common during early than late embryonic period, i.e., from day 8th to 16th at hatching blastocysts initiation elongation commencement implantation without affecting cycle lengths. Early mortality a source with rate up 40%. also reported due mineral deficiency heat stress buffaloes. Physical modifi...

The binding pattern of a rabbit antiserum raised against mouse-ectoplacental-cone trophoblast on component cell populations in the pre-implantation and early post-implantation mouse embryo has been examined at the electron-microscope level using an immunoperoxidase-labelling technique. Binding was not detectable on the 1-cell stage, appeared at low levels at the 8-cell stage and was heavy on th...

Adherence of an embryo to the uterus represents the most critical step of the reproductive process. Implantation is a synchronized event between the blastocyst and the uterine luminal epithelium, leading to structural and functional changes for further embryonic growth and development. The milieu comprising the complex process of implantation is mediated by estrogen through diverse but interdep...

The process of implantation is a 'two-way' interaction between the blastocyst and uterus. It has long been suspected that histamine is an important mediator in embryo-uterine interactions during implantation, but its source, targets and mechanism of actions remained undefined. We have recently demonstrated that uterine epithelial cells are the source of histamine, which peaks on day 4 of pregna...

Morphological evidence on early stages of human implantation is limited to very few sporadic observations. The nature of implantation which requires the presence of both maternal and embryonic tissues, combined with the currently existing ethical constraints on human studies, appear to preclude generation of new data. However, research on relevant animal and in-vitro models as well as studies o...

Successful embryo implantation requires complex interactions between the uterus and embryo, including the establishment of maternal immunologic tolerance of fetal material. The maternal-fetal interface is dynamically populated by a wide variety of innate immune cells; however, the relevance of uterine DCs (uDCs) within the decidua to the success of implantation has remained unclear. In this iss...

In mammals, the development of pluripotency and specification of primordial germ cells (PGCs) have been studied predominantly using mice as a model organism. However, divergences among mammalian species for such processes have begun to be recognized. Between humans and mice, pre-implantation development appears relatively similar, but the manner and morphology of post-implantation development a...

The distribution and intensity of delta 5-3 beta-hydroxysteroid dehydrogenase (3 beta-HSD) activity was examined histochemically in ovaries from Mongolian gerbils during pregnancy and undergoing lactational delay. In all of the ovaries examined the corpora lutea showed the strongest level of activity, but like the interstitium and granulosa, there was no relationship between the enzyme pattern ...

We examined the cytotoxic effects of dillapiole, a phenylpropanoid with antileishmanial, anti-inflammatory, antifungal, and acaricidal activities, on the blastocyst stage of mouse embryos, subsequent embryonic attachment and outgrowth in vitro, and in vivo implantation via embryo transfer. Blastocysts treated with 2.5-10 μM dillapiole exhibited a significant increase in apoptosis and correspond...