We have compared two methods for separating the isoenzymes of creatine kinase in serum and measuring their activity: chromatography on diethylaminoethyl-Sephadex A-50, with both continuous and discontinuous gradient elution, and electrophoresis on cellulose acetate. Results by continuous and discontinuous gradient elution correlated well both for tissue extracts and sera. Electrophoresis on cel...

OBJECTIVE Analysing the fundamental methodological aspects which should be considered when drawing up calibration procedure for electro-medical equipment, thereby permitting international standard-based accreditation of electro-medical metrology laboratories in Colombia. METHODS NTC-ISO-IEC 17025:2005 and GTC-51-based procedures for calibrating electro-medical equipment were implemented and t...

As a part of this study, mathematical model based on two-compartment pharmacokinetic system with general fractional derivatives was developed. Its aim to describe the release BisGMA and TEGDMA monomers from composite core resin over three time periods. For purpose, five Clearfil Photo Core samples were prepared elution measured using high performance liquid chromatography at 1, 7 28 days post-i...

Nisin is an antimicrobial peptide produced and secreted by several L. lactis strains and is specifically active against Gram-positive bacteria. In previous studies, nisin was purified via cation exchange chromatography at low pH employing a single-step elution using 1 M NaCl. Here, we describe an optimized purification protocol using a five-step NaCl elution to remove contaminants. The obtained...

Concentration dependence of elution volume was determined for Blue Dextran 2000, Dextran 500, Dextran sulphate 500 and bovine serum albumin on columns of Sephadex G-100 equilibrated with sodium phosphate buffer, I 0.1, pH6.8. From the results for Dextran 500, it was shown that a linear relation exists between elution volume and the corresponding osmotic pressure calculated for the same concentr...

The basic electro-kinetic process is defined by forced/induced movement of target pollutants in soil/sand medium under applied electric field low strength between electrodes of opposite polarities. There are several specific mechanisms that contribute to this movement of pollutants. However, one study suggested that the most important ones are electro-osmosis, electro migration, and electrophor...

Ion exchange is a very useful technique to separate protein mixtures under mild conditions, most commonly by applying a shallow gradient of increasing salt concentration as eluent. pH gradient elution is used much less frequently, except in specialist applications such as chromatofocusing, and can require complex buffer systems. However, today’s modern quaternary HPLC systems are ideally suited...

Even though antibiotic eluting bone cements are successfully used in orthopedic practice, adding chemotherapy drugs to bone cement pose a different set of problems. This is mainly due to the toxicity concerns associated with the use of chemotherapy agents. To avoid local or systemic toxicity, the drug elution profile of a particular bone cement/drug concentration need to be determined. The drug...

A modified broth-disk elution method for routine susceptibility testing of anaerobic bacteria was compared with the proposed reference agar dilution method under study by the National Committee on Clinical Laboratory Standards. The broth-disk elution method used a 24-h test incubation period, Schaedler broth medium supplemented with menadione, and GasPak (BBL Microbiology Systems) incubation. R...