نتایج جستجو برای: dna repair kinetics

تعداد نتایج: 698452  

Journal: :Physical review letters 2008
Ravindra Venkatramani Ravi Radhakrishnan

High fidelity polymerases are efficient catalysts of phosphodiester bond formation during DNA replication or repair. We interpret molecular dynamics simulations of a polymerase bound to its substrate DNA and incoming nucleotide using a quasiharmonic model to study the effect of external forces applied to the bound DNA on the kinetics of phosphoryl transfer. The origin of the force dependence is...

Journal: :The Journal of biological chemistry 2004
Marc Audebert Bernard Salles Patrick Calsou

The efficient repair of DNA double-strand breaks (DSBs) is critical for the maintenance of genomic integrity. In mammalian cells, the nonhomologous end-joining process that represents the predominant repair pathway relies on the DNA-dependent protein kinase (DNA-PK) and the XRCC4-DNA ligase IV complex. Nonetheless, several in vitro and in vivo results indicate that mammalian cells use more than...

2018
Emily Koury Kailey Harrell Sarit Smolikove

Studies of the repair pathways associated with DNA double strand breaks (DSBs) are numerous, and provide evidence for cell-cycle specific regulation of homologous recombination (HR) by the regulation of its associated proteins. Laser microirradiation is a well-established method to examine in vitro kinetics of repair and allows for live-imaging of DSB repair from the moment of induction. Here w...

2014
Scott Cukras Nicholas Morffy Takbum Ohn Younghoon Kee

Protein neddylation is involved in a wide variety of cellular processes. Here we show that the DNA damage response is perturbed in cells inactivated with an E2 Nedd8 conjugating enzyme UBE2M, measured by RAD51 foci formation kinetics and cell based DNA repair assays. UBE2M knockdown increases DNA breakages and cellular sensitivity to DNA damaging agents, further suggesting heightened genomic in...

Journal: :Journal of visualized experiments : JoVE 2010
F Noah Biro Jie Zhai Christopher W Doucette Manju M Hingorani

Transient kinetic analysis is indispensable for understanding the workings of biological macromolecules, since this approach yields mechanistic information including active site concentrations and intrinsic rate constants that govern macromolecular function. In case of enzymes, for example, transient or pre-steady state measurements identify and characterize individual events in the reaction pa...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 2016
Sheera Adar Jinchuan Hu Jason D Lieb Aziz Sancar

We recently developed a high-resolution genome-wide assay for mapping DNA excision repair named eXcision Repair-sequencing (XR-seq) and have now used XR-seq to determine which regions of the genome are subject to repair very soon after UV exposure and which regions are repaired later. Over a time course, we measured repair of the UV-induced damage of cyclobutane pyrimidine dimers (CPDs) (at 1, ...

Journal: :Journal of virology 1973
K Carlson Z K Lorkiewicz A W Kozinski

Discontinuities of T4 DNA which are caused by excision of UV-damaged areas, by decay of (32)P atoms, or which are present in DNA from rII(-)lig(am) (-) phage produced in a host nonpermissive for amber mutants are all repaired by bacterial enzymes after infection in the presence of chloramphenicol. Escherichia coli DNA polymerase I participates in the host-mediated repair, but an approximately 2...

Journal: :iranian biomedical journal 0
پژمان فرد اصفهانی pezhman fard-esfahani ارمغان فرد اصفهانی armaghan fard-esfahani شیما فیاض shima fayaz بهاره قنبرزاده bahareh ghanbarzadeh پریناز سعیدی parinaz saidi ریحانه محبتی reyhaneh mohabati سید کاظم بیدکی

background: x-ray repair cross-complementing group 1 (xrcc1) gene is a dna repair gene and its non-synonymous single nucleotide polymorphisms (snp) may influence dna repair capacity which has been considered as a modifying risk factor for cancer development. methods: a case-control study was conducted to investigate impact of three frequently studied polymorphisms (arg194trp, arg280his and arg3...

2016
Ran Chen Shyamal Subramanyam Adrian H. Elcock Maria Spies Marc S. Wold

Replication protein A (RPA), the major eukaryotic single-stranded DNA (ssDNA) binding protein, is essential for replication, repair and recombination. High-affinity ssDNA-binding by RPA depends on two DNA binding domains in the large subunit of RPA. Mutation of the evolutionarily conserved aromatic residues in these two domains results in a separation-of-function phenotype: aromatic residue mut...

نمودار تعداد نتایج جستجو در هر سال

با کلیک روی نمودار نتایج را به سال انتشار فیلتر کنید