The endogenous DNA adduct, M(1)dG, has been shown to arise in vitro in reactions of dG with malondialdehyde (MDA), a product of both lipid peroxidation and 4'-oxidation of deoxyribose in DNA, and with base propenals also derived from deoxyribose 4'-oxidation. We now report the results of cellular studies consistent with base propenals, and not MDA, as the major source of M1dG under biological c...

Reactivities of chromium compounds with DNA were investigated by the DNA sequencing technique using 32P 5'-end-labeled DNA fragments, and the reaction mechanism was investigated by ESR spectroscopy. Incubation of double-stranded DNA with sodium chromate(VI) plus hydrogen peroxide or potassium tetraperoxochromate(V) led to the cleavage at the position of every base, particularly of guanine. Even...

Radikal bebas merupakan suatu molekul yang sangat reaktif dan tidak stabil, dapat mengakibatkan kerusakan protein, Deoxyribose Nucleic Acid (DNA), membran sel, serta memicu timbulnya penyakit degeneratif. Kerusakan-kerusakan tersebut dicegah dengan senyawa antioksidan alami maupun sintetik. Caulerpa sp. rumput laut hijau berpotensi sebagai sumber alami. umum diolah menjadi urap dalam keadaan se...

Although several methods (1-5) are used for the determination of deoxyribonucleic acid, none is without some limitations. For example, procedures (1) that depend on the analysis of phosphate in fractionated materials are of limited sensitivity. The use of the absorption at 260 rnp as a measure of deoxyribonucleic acid (2) does not provide a procedure giving readily comparable values for differe...

Vertical excitation energies for DNA and RNA nucleosides are determined with electron structure calculations using the time-dependent density functional theory (TDDFT) method at the B3LYP/6-311++G(d,p) level for nucleoside structures optimized at the same level of theory. The excitation energies and state assignments are verified using B3LYP/aug-cc-pVDZ level calculations. The nature of the fir...