Effects of serum and protein on assays measuring aspartate aminotransferase (L-aspartate: 2-oxoglutarate aminotransferase, EC 2.6.1.1) were examined. Increasing amounts of normal human serum or human serum albumin in samples with fixed amounts of purified enzyme decrease absorbance values and thus distort the results obtained in an assay in which diazonium-salt chromogenic reagent is used. Seru...

Abstract Decarboxylation of cannabidiolic acid (CBDA) is an important step for efficient production the active pharmaceutical component cannabidiol (CBD) in Cannabis species. Acidic cannabinoids (ACBs) can be easily transformed into neutral via loss carbon dioxide when exposed to heat. During thermal process, several products including psychotropic ? 9 -tetrahydrocannabinol ( -THC) and its isom...

BACKGROUND P450 fatty acid decarboxylases represented by the unusual CYP152 peroxygenase family member OleTJE have been receiving great attention recently since these P450 enzymes are able to catalyze the simple and direct production of 1-alkenes for potential applications in biofuels and biomaterials. To gain more mechanistic insights, broader substrate spectra, and improved decarboxylative ac...

The synergistic effect Ni–Cu in the bimetallic catalyst Ni–Cu/C improved stability and reduction temperature as well enhanced catalytic activity for decarboxylation of palmitic acid.

Three allelic mutants of Arabidopsis thaliana which lack mitochondrial serine transhydroxymethylase activity due to a recessive nuclear mutation have been characterized. The mutants were shown to be deficient both in glycine decarboxylation and in the conversion of glycine to serine. Glycine accumulated as an end product of photosynthesis in the mutants, largely at the expense of serine, starch...

The concentration of indoleacetic acid (IAA) in plant tissues is regulated, in part, by its rate of decarboxylation. However, the commonly used in vitro assays for IAA oxidase may not accurately reflect total in vivo decarboxylation rates. A method for measuring in vivo decarboxylation was utilized in which (14)CO(2) is collected following uptake of [1-(14)C]IAA by excised tissue sections. Afte...

The nonoxidative decarboxylation of aromatic acids occurs in a range of microbes and is of interest for bioprocessing and metabolic engineering. Although phenolic acid decarboxylases provide useful tools for bioindustrial applications, the molecular bases for how these enzymes function are only beginning to be examined. Here we present the 2.35-Å-resolution X-ray crystal structure of the feruli...

Peroxidase, present in both neutrophils and eosinophils, is thought to be involved in the bactericidal activity of leukocytes, through either the decarboxylation of amino acids in the presence of chloride or the iodination of proteins in the presence of iodide. We examined these activities in sonic extracts of purified neutrophils and eosinophils and related them to the ability of the extracts ...

Electrochemical decarboxylation of acetic acid on boron-doped diamond electrodes (BDD) was studied as a possible means to decrease the acidity pyrolysis oil. It is shown that occurs without...

Renal arginase activity is a potent source of ornithine (Orn) for polyamine synthesis. Ornithine decarboxylase (ODC) was localized along the mouse and rat nephron by incubating viable nephron segments isolated by microdissection from collagenase-treated kidneys with or withoutd,l-2-(difluoromethyl)ornithine (DFMO), a selective inactivator of ODC. Tubules from either control or DFMO-treated anim...