نتایج جستجو برای: core protein

تعداد نتایج: 1424777  

Journal: :Proteins 1997
H K Choi G Lu S Lee G Wengler M G Rossmann

Alphaviruses are enveloped, insect-borne viruses, which contains a positive-sense RNA genome. The protein capsid is surrounded by a lipid membrane, which is penetrated by glycoprotein spikes. The structure of the Sindbis virus (SINV) (the type virus) core protein (SCP) was previously determined and found to have a chymotrypsin-like structure. SCP is a serine proteinase which cleaves itself from...

Journal: :Chemical communications 2010
Yiquan Wu I-Chien Liao Scott J Kennedy Jinzhi Du Jun Wang Kam W Leong Robert L Clark

This communication describes a single-step electrospraying technique that generates core-shell microspheres (CSMs) with encapsulated protein as the core and an amphiphilic biodegradable polymer as the shell. The protein release profiles of the electrosprayed CSMs showed steady release kinetics over 3 weeks without a significant initial burst.

2017
Yu-Yun Shao Min-Shu Hsieh Han-Yu Wang Yong-Shi Li Hang Lin Hung-Wei Hsu Chung-Yi Huang Chih-Hung Hsu Ann-Lii Cheng

Increased angiogenic activity has been demonstrated in hepatitis C virus (HCV)-related hepatocellular carcinoma (HCC), but the mechanism was unclear. To study the role of HCV core protein, we used tube formation and Matrigel plug assays to assess the proangiogenic activity of an HCC cell line, HuH7, and 2 of its stable clones-HuH7-core-high and HuH7-core-low, with high and low HCV core protein ...

2010
Pornpun Vivithanaporn Ferdinand Maingat Liang-Tzung Lin Hong Na Christopher D. Richardson Babita Agrawal Éric A. Cohen Jack H. Jhamandas Christopher Power

BACKGROUND Hepatitis C virus (HCV) genomes and proteins are present in human brain tissues although the impact of HIV/HCV co-infection on neuropathogenesis remains unclear. Herein, we investigate HCV infectivity and effects on neuronal survival and neuroinflammation in conjunction with HIV infection. METHODOLOGY Human microglia, astrocyte and neuron cultures were infected with cell culture-de...

Journal: :The Journal of biological chemistry 2005
Masaaki Korenaga Ting Wang Yanchun Li Lori A Showalter Tehsheng Chan Jiaren Sun Steven A Weinman

Hepatitis C infection causes a state of chronic oxidative stress, which may contribute to fibrosis and carcinogenesis in the liver. Previous studies have shown that expression of the HCV core protein in hepatoma cells depolarized mitochondria and increased reactive oxygen species (ROS) production, but the mechanisms of these effects are unknown. In this study we examined the properties of liver...

Journal: :Journal of virology 2003
Kohji Moriishi Tamaki Okabayashi Kousuke Nakai Kyoji Moriya Kazuhiko Koike Shigeo Murata Tomoki Chiba Keiji Tanaka Ryosuke Suzuki Tetsuro Suzuki Tatsuo Miyamura Yoshiharu Matsuura

Hepatitis C virus (HCV) core protein plays an important role in the formation of the viral nucleocapsid and a regulatory protein involved in hepatocarcinogenesis. In this study, we have identified proteasome activator PA28gamma (11S regulator gamma) as an HCV core binding protein by using yeast two-hybrid system. This interaction was demonstrated not only in cell culture but also in the livers ...

Journal: :ChemBioChem 2023

Seek a cell anchorage galectin bridge: Transforming the wildtype Galectin (Gal)-1 and -3 to tandem-repeat type variant via rational protein engineering enhanced cis-binding activity with O-Mannosylated core M1 glycopeptides of α-dystroglycan (DG). Furthermore, Gal-1 variants (but not Gal-3 variants) demonstrated trans-bridging prepared α-DG core-M1 glycoconjugates laminins in situ carbohydrate-...

Journal: :Nature communications 2016
Sayaka Aizawa Toru Okamoto Yukari Sugiyama Takahisa Kouwaki Ayano Ito Tatsuya Suzuki Chikako Ono Takasuke Fukuhara Masahiro Yamamoto Masayasu Okochi Nobuhiko Hiraga Michio Imamura Kazuaki Chayama Ryosuke Suzuki Ikuo Shoji Kohji Moriishi Kyoji Moriya Kazuhiko Koike Yoshiharu Matsuura

Signal-peptide peptidase (SPP) is an intramembrane protease that participates in the production of the mature core protein of hepatitis C virus (HCV). Here we show that SPP inhibition reduces the production of infectious HCV particles and pathogenesis. The immature core protein produced in SPP-knockout cells or by treatment with an SPP inhibitor is quickly degraded by the ubiquitin-proteasome p...

Journal: :The Journal of Experimental Medicine 1985
A J Sant S E Cullen K S Giacoletto B D Schwartz

The murine Ia-associated chondroitin sulfate proteoglycan (CSPG) was studied both biochemically and immunochemically to determine the nature of its core protein. Chondroitinase ABC or chondroitinase AC treatment of the CSPG digested the chondroitin sulfate glycosaminoglycan, yielding a core protein that migrated with an apparent molecular weight of 38,000. Comparative V8 protease digestion of t...

Journal: :Journal of virology 1999
T Shimoike S Mimori H Tani Y Matsuura T Miyamura

To clarify the binding properties of hepatitis C virus (HCV) core protein and its viral RNA for the encapsidation, morphogenesis, and replication of HCV, the specific interaction of HCV core protein with its genomic RNA synthesized in vitro was examined in an in vivo system. The positive-sense RNA from the 5' end to nucleotide (nt) 2327, which covers the 5' untranslated region (5'UTR) and a par...

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