the natural lignan podophyllotoxin, a dimerized product of two phenylpropanoid moieties which occurs in a few plant species, is a pharmacologically important compound for its anticancer activities. optimization of the in vitro cultures might lead to a renewable source of lignans for medicinal uses. in order to maximize efficiency of podophyllotoxin accumulation via in vitro cultures, the effect...

In this paper a new nanostructured support for the culture of cells is presented. The support consists of fields of sharp and high-aspect-ratio nanoneedles. The support is obtained through a specifically developed process that allows controlling the nanoneedles’ densities and height. The nanoneedles are typically 10 μm high with tip diameters under 200 nm. Cell viability on this support was eva...

THis careful and detailed history of neuro-anatomical thinking sets present-day concepts in their historical context and fulfils a useful function. As one might expect from the title of the book, the subdivisions are anatomical and the author reviews descriptions of the basal ganglia and diencephalon, the 'olfactory brain' and the cerebral convolutions and fissures from the time of Galen to the...

backgrounds: evidence is accumulating to support disruption of tissue architecture as a powerful event in tumor formation. for the past four decades, intensive cancer research with the premise of “cancer as a cell based-disease” focused on finding oncogenes or tumor suppressor genes. however, the role of the tissue architecture was neglected. three dimensional (3d) cell cultures which can recap...

This book is a collection of papers dealing with one of the most ubiquitous and troublesome nuisances of present day research in cell biology. Mycoplasma contamination of cell lines in a cell culture laboratory can cause changes in growth and biochemical properties of the cells, thus introducing uncontrolled variables into the experimental system. The papers in this book were presented at a wor...

the objective was to investigate the presence of mycoplasma and chlamydia in the synovial fluid of patients with rheumatoid arthritis (ra) and other chronic arthritides. samples of synovial fluid (sf) were collected from all patients presenting with an articular effusion. seventy sf samples were subjected to study for mycoplasma and cultured on standard media for mycoplasma. the 70 other sf sam...

Background: Cell-based treatment approach using differentiated mesenchymal stem cells (MSCs) and mature chondrocytes has been considered as an advanced treatment for cartilage repair. We investigated the differentiated level of these two cell types that is crucial for their repair capacity for cartilage defect at a co-culture micro mass system. Methods: Passaged-2 MSCs isolated from the mouse b...

due to the worldwide increase in the number of older people in both developed and developing countries, there is a public health concern for dealing with age related diseases such as neurodegenerative diseases. there is little known about the difference in neuronal cell responses between genders. our understanding of the neuronal cell response regarding genders, will be useful for developing mo...