The ALP1 [aci-reductone dioxygenase (ARD)-like protein 1] gene was identified in a comprehensive cDNA subtraction aimed at identifying genes regulated by androgens in the rat ventral prostate. ALP1 is homologous to the ARD/ARD' that were discovered in Klebsiella pneumoniae as enzymes that have the same polypeptide sequence and differ only in their metal content. This family of proteins is evolu...

The paper reports a 5 year old male sheep-goat interspecific hybrid born under natural conditions. The hybrid was castrated at 10 months of age. Karyotype analysis confirmed that the animal was a male and a hybrid with 57 chromosomes, an intermediate number between sheep (2n=54) and goat (2n=60). Morphological characteristics of the animal were compared with those of castrated male Tswana goats...

Estrogen 2and 4-hydroxylase (ESH), a microsomal enzyme which mediates the formation of catechol estrogens, has been studied in the kidneys of castrated male Syrian hamsters, a species uniquely susceptible to induction of renal carcinomas by both steroidal and stilbene estrogens. The apparent K„, for estrone was 17.0 MM,and V,™«, was 0.5 pmol per mg protein per min for ESH in renal microsom...

The present study characterized the in vitro androgen receptor (AR) binding affinity, in vitro and in vivo pharmacological activity, and in vivo pharmacokinetics and metabolism of acetothiolutamide, a nonsteroidal AR ligand. AR binding was determined by a competitive binding assay. In vitro AR agonist activity was examined by a cotransfection assay. Acetothiolutamide displayed high AR binding a...

The results of several investigations1-5 have suggested that nucleic acids play a role in mediating the anabolic action of androgens. RNA extracted from prostatic nuclei of androgen-treated castrates enhanced the incorporation of amino acids into protein in a bacterial ribosomal system.6 An RNA preparation from the uteri of estrogen-treated rats induced morphologic changes resembling those prod...

Objective: This study was conducted to investigate the effects of estradiol benzoate injection to intact and castrated male rabbits on LH, FSH, testosterone and prostate tissue. Materials and methods: A total of 72 mature male rabbits were used in this study. The rabbits were randomly divided into two groups (36 intact and 36 castrated) and each group was further divided into four sub-groups. T...

PURPOSE The current investigators have shown that androgen treatment suppresses inflammation and stimulates the function of lacrimal glands in mouse models of Sjögren's syndrome. Recently, others have hypothesized that androgen insufficiency induces an autoimmune process in lacrimal tissue, leading to inflammation, a Sjögren's syndrome-like pathology, and aqueous tear deficiency. The purpose of...

background: this study aimed to assess follicle survival after xenotransplantation of sheep ovarian tissue into male and female immunodeficient rats. we evaluated the effects of gonadotropin treatment on follicular development in the transplanted tissue. materials and methods: in this experimental study, sheep ovarian cortical strips were transplanted into the neck back muscles of 8 male and 8 ...

MATERIALS AND METHODS The adrenal cortical tumors used, all arising in castrated males and, if already transplanted, being carried in castrated males (18), were: CE 1, the original neoplasm in a CE mouse; BALE 5880/1,1 a first-generation subcutaneous transplant in a BALB/c; B ALB 5880/2, a second-generation sub cutaneous transplant of the same tumor in a BALB/c; and CE 366/2, a second-generatio...