In the present study, an efficient protocol has been developed for callus induction and production of RA in callus culture of Satureja khuzistanica for the first time. In vitro callus induction was achieved from young shoot tip explants cultured on MS and B5 media supplemented with different concentrations of IBA (0.1, 1.0, 2.0 and 5.0 mgl-1) solely or in combination with cytokinins BAP and KIN...

Genetic improvement of garlic through conventional breeding is very difficult due to sterile nature of its flower. Hence, an alternative system is desirable to induce genetic variation. Tissue culture could be a good opportunities and somatic embryogenesis is one of the potential techniques of tissue culture for in vitro regeneration of garlic plant. The successes and production of somatic embr...

The antagonistic interaction between the grass herbicide, diclofopmethyl (methyl 2-[4(2',4'-dichlorophenoxy)phenoxy]propanoate) (DM), and 2,4-dichlorophenoxyacetic acid (2,4-D), was demonstrated in DM-resistant soybean (Glycine max [L.] Merr.) and DM-susceptible corn (Zea mays L.). 2,4-D caused root shortening and thickening, and induced callus growth in soybean and corn root tissue cultures at...

Lakum plant (Causonis trifolia (L.) Mabb. & J.Wen) contains secondary metabolites with potential medicinal ingredients. It can treat various diseases, such as antidiabetic, antibacterial, antiprotozoal, antitumor, and anti-cancer. Propagation production of in plants be carried out vitro through callus culture are influenced by the concentration growth regulators. This study aimed to determi...

in the present study, an efficient protocol has been developed for callus induction and production of ra in callus culture of satureja khuzistanica for the first time. in vitro callus induction was achieved from young shoot tip explants cultured on ms and b5 media supplemented with different concentrations of iba (0.1, 1.0, 2.0 and 5.0 mgl-1) solely or in combination with cytokinins bap and kin...

the present investigation was conducted to study the effects of type and concentration of auxin and cytokinin on callus induction of three strawberry (kurdistan, parose and camarosa) cultivars. for this purpose, leaf blade, nodal, petiole, receptacle, stamen and flower bud explants were cultured on murashige and skoog (ms) medium at three separate experiments included 2, 4 dichlorophenoxyacetic...

In the plant regeneration process, a great deal of variability is observable between different varieties and genotypes. Even within the same genotype, there are differences in the intensity of this process. New biologically active substances are currently being tested to increase the effectiveness of regeneration in plants. Among them are zearelonene (ZEN) – a substance obtained from an extract...

Phytosulfokine (PSK), a 5-amino acid sulfated peptide that has been identified in conditioned medium of plant cell cultures, promotes cellular growth in vitro via binding to the membrane-localized PSK receptor. Here, we report that loss-of-function and gain-of-function mutations of the Arabidopsis (Arabidopsis thaliana) PSK receptor gene (AtPSKR1) alter cellular longevity and potential for grow...

The natural lignan podophyllotoxin, a dimerized product of two phenylpropanoid moieties which occurs in a few plant species, is a pharmacologically important compound for its anticancer activities. Optimization of the in vitro cultures might lead to a renewable source of lignans for medicinal uses. In order to maximize efficiency of podophyllotoxin accumulation via in vitro cultures, the effect...

-Callus derived from different plant parts of various strains of Cannabi8 8ativa L. was established and analyzed for growth on different media and for can­ nabinoid content by thin-layer (tic) and gas-liquid chromatography (glc). Plant organs which included bracts and anther-calyx complexes of drug (152) and fiber (150) types and maturing leaves of Mexican (drug) and Turkish (fiber) strains wer...