0.1% Protamine sulphate in normal (0.85% w/v) saline has been used as a diluent in Brucella serology instead of saline as such. The use of protamine sulphate in this concentration has obviated the need for performing the modified Coombs test to detect non-agglutinating Brucella antibodies. This method has been used in the Microbiological Diagnostic Unit of the University of Melbourne in the rou...

Mice passively immunized prior to a challenge infection with immunoglobulin G (IgG) and IgM monoclonal antibodies (MAbs) specific for a common epitope of both A- and M-dominant strains had viable Brucella abortus 544 or Brucella melitensis H38 counts in the spleen reduced to the same extent as did mice passively immunized with MAbs specific for either the A or the M epitope. The IgA MAb was not...

Abstract Background Brucellosis is a serious zoonosis disease that frequently causes significant economic loss in animal husbandry and threatens human health. Therefore, we established rapid, accurate, simple sensitive fluorescent immunochromatographic strip test (ICST) based on quantum dots (QDs) for detection the antibodies of Brucella infection animals serum. Results The strips were successf...

BACKGROUND Brucellosis is an endemic zoonotic disease in the Middle East. This study intended to design a uniplex PCR assay for the detection and differentiation of Brucella at the species level and determining the antibiotic susceptibility pattern of Brucella in Iran. METHODS Sixty-eight Brucella specimens (38 animal and 30 human specimens) were analyzed using PCR (using one pair of primers)...

By immunizing mice with killed whole bacterial cells of Brucella abortus S (99), a panel of six hybridomas producing monoclonal antibodies (mAb) specific for the surface antigens of this bacterium were produced. ELISA was used to screen the hybridoma supernatants. Immunoblots of the cell extract indicated that three mAb were specific for S-LPS (Ba-1, Ba-2, Ba-3) and three others were reactive w...

Aim: To assess the status of bovine brucellosis in an organized dairy with a past history of Brucella abortions and where Brucella control measures including test and removal, calf-hood vaccination (already present adult animals were not vaccinated), use of semen obtained from a screened bull and general hygienic measures helps in the control of brucellosis in the farm have been implemented for...

Bovine Brucellosis is a serious disease of livestock that has significant animal, public health and international trade consequences. Considering the damage done by the infection in animals such as death losses, decreased milk production, weight loss, infertility and lameness, this disease is a formidable threat to livestock. The fact that this disease can spread rapidly and be transmitted to h...

The microaerophilic nature of Campylobacter fetus has complicated its recovery from human and animal sources. In this study, modifications of brucella agar and broth were tested for enhancement of growth and aerotolerance of 64 strains of C. fetus, representing each subspecies. Brucella agar supplemented with 0.025% each FeSO4 7H2O, sodium metabisulfite, and sodium pyruvate, supported growth of...

No data are available concerning the seroprevalence of brucellosis in Central African Republic (CAR) and the last report concerning the seroprevalence of Q fever in CAR is from 1995. The aim of our study was to determine the prevalence of these diseases in CAR, especially in Zebu cattle. We used the Rose Bengal Plate Test to test 2032 bovine serum samples for antibodies to Brucella spp. and an ...

A field trial was conducted in a camel brucellosis-free herd to evaluate antibody response to the Brucella melitensis Rev.1 vaccine in camels and assess shedding of the vaccine strain in milk. Twenty eight camels were divided into four groups according to their age and vaccination route. Groups A (n=3) and B (n=3) consisted of non-pregnant lactating female camels, vaccinated through subcutaneou...