Bioluminescence (BL) predictability experiments (predictions of the intensity, depth, and distance offshore of the BL maximum) were conducted using an advective–diffusive tracer model with velocities and diffusivities from a fine-resolution model of the Monterey Bay, California, area. For tracer initialization, observations were assimilated into the tracer model while velocities and diffusiviti...

Bioluminescence imaging with luciferase enzymes and luciferin small molecules is a well-established technique for tracking cells and other biological features in rodent models. Despite its popularity, bioluminescence has long been hindered by a lack of distinguishable probes. Here we present a method to rapidly identify new substrate-selective luciferases for multicomponent imaging. Our strateg...

Herein a convenient strategy was developed to quantify protein SUMOylation. The amount of target proteins and SUMO proteins conjugated to them could be quantified through luciferase-based bioluminescence detection and the relative bioluminescence was used to evaluate the SUMOylation degree of the target proteins.

Compartmentalization of specialized functions to discrete locales is a fundamental theme of eucaryotic organization in cells. We report here that bioluminescence of the dinoflagellate alga Gonyaulax originates in vivo from discrete subcellular loci that are intrinsically fluorescent. We demonstrate this localization by comparing the loci of fluorescence and bioluminescence as visualized by imag...

The capability of animals to emit light, called bioluminescence, is considered to be a major factor in ecological interactions. Because it occurs across diverse taxa, measurements of bioluminescence can be powerful to detect and quantify organisms in the ocean. In this study, 17 years of video observations were recorded by remotely operated vehicles during surveys off the California Coast, from...

The study addresses biological effects of low-dose gamma-radiation. Radioactive 137Cs-containing particles were used as model sources of gamma-radiation. Luminous marine bacterium Photobacterium phosphoreum was used as a bioassay with the bioluminescent intensity as the physiological parameter tested. To investigate the sensitivity of the bacteria to the low-dose gamma-radiation exposure (≤250 ...

A significant portion of bioluminescence in all oceans is produced by dinoflagellates. Numerous studies have documented the ubiquitous distribution of bioluminescent dinoflagellates in near surface waters (Seliger et al., 1961; Yentsch and Laird 1968; Tett 1971; Tett and Kelly 1973). The number of bioluminescent species and their relative abundance change temporally, with depth, and geographica...