Differential display technique was applied in order to identify transcripts which are present in axenic amastigotes but not in promastigotes of the Leishmania panamensis parasites. One of them was cloned and the sequence reveals an open reading frame of 364 amino acids (approximately 40 kDa). The deduced protein is homologous to the serine/threonine protein kinases and specially to the mitogen ...

Four species of Naegleria were tested for their ability to migrate under agarose. Pathogenic N. fowleri strains exhibited rapid locomotion at 37 degrees C. Environmental isolates of N. fowleri moved faster than clinical isolates which had been kept in axenic culture for longer periods, and this result was confirmed by using the 84-2205-7 strain kept in axenic culture for 1 or 5 months. Nonpatho...

We report here the 3,586,065-bp draft genome of Geobacillus sp. LEMMY01, which was isolated (axenic culture) from a thermophilic chemolitoautotrophic consortium obtained from the site of a burning grass pile. The genome contains biosynthetic gene clusters coding for secondary metabolites, such as terpene and lantipeptide, confirming the biotechnological potential of this strain.

Although many isolation techniques for algaeaxenic cultures of algae are known, successful approaches species and contaminant-specific. The commercially important alga Haematococcus lacustris has been intensively studied due to its natural production the high-value carotenoid astaxanthin, yet there no reports axenic H. cultures. In this article, we describe lacustris, originally isolated in non...

Leishmaniasis is a tropical parasitic infection. The resistance and toxicity issues are the major complications and remain significant consequences related to the treatment of leishmaniasis with the recent and classical drugs. Thus there is an immediate requirement to develop new compounds for the treatment of this protozoan disease. Sea cucumbers or holothurians are potentially presented as th...

Axenic gametes of the marine green macroalga Ulva mutabilis Føyn (Ria Formosa, locus typicus) exhibit abnormal development into slow-growing callus-like colonies with aberrant cell walls. Under laboratory conditions, it was previously demonstrated that all defects in growth and thallus development can be completely abolished when axenic gametes are inoculated with a combination of two specific ...

Oxyrrhis marina Dujardin is a non-photosynthetic dinoflagellate whose obvious mode of nutrition is phagotrophy. The method of feeding was described by Barker (1935). It is an extremely euryhaline and hardy organism and is apparently very successful in brackish habitats such as supralittoral rock pools and ditches (Droop, 1953a). Owing to the ease with which it can be cultivated Oxyrrhis is a ch...

The differences in fragility of microorganisms when treated in the French press are useful for destroying bacteria and mold contaminants, and assist in the preparation of axenic cultures of Chlorella.

1. A simple axenic medium suitable for the growth of the myxamoebae of a strain of the cellular slime mould Dictyostelium discoideum is described. 2. Procedures suitable for the growth of this strain in liquid and on solid media are described. 3. Conditions suitable for initiating the cell differentiation of myxamoebae grown axenically are described.