This paper describes the application programming interface (API) providing authorization and access control in IRO-DB. IRO-DB is an ODMB compliant federated database system supporting interoperable access between relational and object-oriented databases. The developed security API implements a federated, administrative, discretionary access control policy which is role-based but additionally su...

Many isolates of glucose-nonfermenting gram-negative bacilli (NFB) cultured from clinical veterinary specimens are not identified because of the large number of identification tests required. We evaluated two commercial identification systems to determine if they could accurately identify NFB isolated from animals. Of 182 strains of NFB, the MicroScan Urinary Combo Panel (MicroScan, Inc., Campb...

This study explores the possibility of combining the BacT/Alert Microbial Detection System with the VITEK 2 system to achieve rapid bacterial identification and susceptibility testing. Direct inoculation of bacterial suspension to the VITEK 2 ID-GNB card and AST-NO09 card was made by differential centrifugation of blood cultures of organisms with gram-negative enteric bacillus-like morphology. ...

Fifty serotyped isolates each of Pasteurella multocida and Pasteurella haemolytica were tested on the API 20E strip (Analytab Products, Plainview, N.Y.), the Oxi/Ferm tube (Roche Diagnostics, Nutley, N.J.), and the Minitek system (BBL Microbiology Systems, Cockeysville, Md.). None of the rapid test systems reliable identified these organisms. With the API system, discrepancies between expected ...

AIMS To ascertain the clinical relevance of a strain of Enterobacteriaceae isolated from the stool of a bone marrow transplant recipient with diarrhoea. The isolate could not be identified to the genus level by conventional phenotypic methods and required 16S ribosomal RNA (rRNA) gene sequencing for full identification. METHODS The isolate was investigated phenotypically by standard biochemic...

Six dairy products were tested. Bifidobacteria were enumerated and isolated using TPY agar modified by the addition of mupirocin (100 mg l). Isolates were identified to the genus level by the detection of fructose-6-phosphate phosphoketolase (F6PPK) and by the FISH method. Bifidobacteria were characterised using API 50 CHL and API ID 32 A Rapid tests. In addition, grow at 46 °C was tested. Subs...

OBJECTIVE To study the in-vitro antimicrobial activity of Cefpirome: A new fourth generation Cephalosporin in comparison with other agents against clinically significant Gram-negative and Gram-positive bacteria. SETTING A multi-center in-vitro study was conducted in 13 centers. MATERIALS AND METHODS Bacterial isolates--A total of 1300 isolates were collected from different clinical laborato...

Ready-to-eat foods have become a global concern because they are source of income for low and middle-income people. Also, constitute the main food students lower to middle-level workers. However, lack knowledge about good processing practices results in microbial contamination that interferes with human health. Enterobacter Cloacae is one most common bacterial contaminants found ready-to-eat ma...

Phyllosphere microbial communities are highly diverse and have important ecological implications; in that context, bacterial identification based on 16S rRNA genes is an important research issue. In studies of phyllosphere microbial communities, microporous filtration and centrifugation are used to collect microorganism samples, but it is unclear which one has a better collection efficiency. In...

the objective of this study was to evaluate lactic flora identification in fresh (one-day) and ripened (90-day) koozeh cheese samples as one of the raw milk cheeses. in first step, in order to isolate the different genera of lactic acid bacteria (lab), selective and differential (corresponding) media were used as follow: mrs for lactobacilli and pediococci, mrs+ vancomycin for leuconostocs, m17...